Catalog Products » Antibodies » antibody : PU.1 Antibody, pAb, Rabbit
Cat. No.
Name
Size   
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Selected
A01692-40
40 μg
$79 $47
PU.1 Antibody, pAb, Rabbitcustom antibody production
PU.1 Antibody, pAb, Rabbit
Full Name PU.1 Antibody, pAb, Rabbit
Abbreviated name-1
PU.1 Antibody, pAb, Rabbit; Spi1 antibody
Documents
PU.1 Antibody, pAb, RabbitDocument-MSDS: 13008_20100629032218.PDF (PDF)
Figures
PU.1 Antibody, pAb, Rabbit zoomPU.1 Antibody, pAb, Rabbit
PU.1 Antibody, pAb, Rabbit zoomPU.1 Antibody, pAb, Rabbit
Description
PU.1 known as Spi1 and Sfpi1, is an ETS-domain transcription factor essential for the development of myeloid and B-lymphoid cells. In myeloid cells, PU.1 may regulate transcription of both FMS and CD18/11B proteins that are central to the osteoclast phenotype. The failure to express PU.1 might preclude development of osteoclasts, leading to arrested bone resorption and osteopetrosis. PU.1 expression increases with the induction of osteoclastogenesis by either 1,25-dihydroxyvitamin D3 or dexamethasone which confirms the association between PU.1 and osteoclast differentiation.
GenScript PU.1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human PU.1 protein (Swiss Prot: P17947).
Ig Subclass
IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript PU.1 Antibody, pAb, Rabbit detects human PU.1 protein.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide from C-terminal of human PU.1 (Swiss Prot: P17947)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml Other Applications: user optimized
Conjugation
Unconjugated
Species Reactivity
Human. Reactivity to other species has not been tested yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 13861_20110906232447.PDF
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