Catalog Products » Antibodies » antibody : PU.1 Antibody, pAb, Rabbit
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Cat. No.
40 μg
PU.1 Antibody, pAb, Rabbit
Full Name PU.1 Antibody, pAb, Rabbit
Abbreviated name-1
PU.1 Antibody, pAb, Rabbit; Spi1 antibody
PU.1 Antibody, pAb, RabbitDocument-MSDS: 13008_20100629032218.PDF (PDF)
PU.1 Antibody, pAb, Rabbit zoomPU.1 Antibody, pAb, Rabbit
PU.1 Antibody, pAb, Rabbit zoomPU.1 Antibody, pAb, Rabbit
PU.1 known as Spi1 and Sfpi1, is an ETS-domain transcription factor essential for the development of myeloid and B-lymphoid cells. In myeloid cells, PU.1 may regulate transcription of both FMS and CD18/11B proteins that are central to the osteoclast phenotype. The failure to express PU.1 might preclude development of osteoclasts, leading to arrested bone resorption and osteopetrosis. PU.1 expression increases with the induction of osteoclastogenesis by either 1,25-dihydroxyvitamin D3 or dexamethasone which confirms the association between PU.1 and osteoclast differentiation.
GenScript PU.1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human PU.1 protein (Swiss Prot: P17947).
Ig Subclass
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
GenScript PU.1 Antibody, pAb, Rabbit detects human PU.1 protein.
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
KLH-coupled synthetic peptide from C-terminal of human PU.1 (Swiss Prot: P17947)
Purification Method
Immunoaffinity chromatography
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml Other Applications: user optimized
Species Reactivity
Human. Reactivity to other species has not been tested yet.
Host Species
Antigen Species
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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