| Full Name |
CD8 Antibody, mAb, Mouse
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Abbreviated name-1 |
CD8 Antibody, mAb, Mouse; |
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Description |
CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck. |
Ig Subclass |
IgG1 |
Storage |
GenScript CD8 Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 4-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript CD8 Antibody, mAb, Mouse recognizes human CD8 antigen, a 32kD transmembrane protein present in the cytotoxic/suppressor T-lymphocyte or on the cell membrance of sinus-lining cells in human spleen. Approximately 80% of T-cells in bone marrow and gut epithelium react with this antibody. |
Concentration |
1 mg/ml, lyophilized in 0.01 M phosphate buffered saline and 0.01 M Tris-Gly buffer, pH 7.4, containing 15 mM sodium azide |
Purification Method |
Protein G affinity purified immunoglobulin |
Applications |
FACS: 1-2 μg/ml
ELISA/Immunoblotting: 1-2 μg/ml
Immunocytochemistry (frozen tissue): 10 μg/ml |
Conjugation |
Unconjugated |
Species Reactivity |
Human. Reactivity to other species is not tested yet. |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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