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CD8 Antibody, mAb, Mouse

*This product has been discontinued! *

Abbreviated name-1 CD8 Antibody, mAb, Mouse;
Description CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
Host Species Mouse
Conjugation Unconjugated
Purification Method Protein G affinity purified immunoglobulin

Formulation 1 mg/ml, lyophilized in 0.01 M phosphate buffered saline and 0.01 M Tris-Gly buffer, pH 7.4, containing 15 mM sodium azide
Clone ID A01697.01
Ig Subclass IgG1
Specificity GenScript CD8 Antibody, mAb, Mouse recognizes human CD8 antigen, a 32kD transmembrane protein present in the cytotoxic/suppressor T-lymphocyte or on the cell membrance of sinus-lining cells in human spleen. Approximately 80% of T-cells in bone marrow and gut epithelium react with this antibody.
Storage GenScript CD8 Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 4-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

FACS: 1-2 μg/ml
ELISA/Immunoblotting: 1-2 μg/ml
Immunocytochemistry (frozen tissue): 10 μg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

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