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*This product is under development*
CD16 Antibody, mAb, Mousecustom antibody production
CD16 Antibody, mAb, Mouse
Full Name CD16 Antibody, mAb, Mouse
Abbreviated name-1
CD16 Antibody, mAb, Mouse;
Documents
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Reference
Description
CD16 is a cluster of differentiation found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages[1]. It can be used to isolate populations of these cells by antibodies directed towards CD16, utilising FACS (fluorescent-activated cell sorting) or MACS (magnetic-activated cell sorting). CD16 has been identified as Fc receptors FcγRIIIa and FcγRIIIb. These receptors bind to the Fc portion of IgG antibodies.
Ig Subclass
IgG1
Storage
GenScript CD16 Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 4-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript CD16 Antibody, mAb, Mouse recognizes human CD16 antigen/Fc Gamma receptor III, a 50-70 kD glycoprotein expressed on NK cells, macrophages, and granulocytes.
Concentration
1 mg/ml, lyophilized in 0.01 M phosphate buffered saline and 0.01 M Tris-Gly buffer, pH 7.4, containing 15 mM sodium azide
Purification Method
Protein G affinity purified immunoglobulin
Applications
FACS: 1-2 μg/ml
ELISA/Immunoblotting: 1-2 μg/ml
Immunocytochemistry (frozen tissue): 10 μg/ml
Conjugation
Unconjugated
Species Reactivity
Human. Reactivity to other species is not tested yet.
Host Species
Mouse
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
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