Catalog Products » Antibodies » THE™ Elite Antibodies » THETM GFP Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A01704-200
200 μg
$741
A01704-100
100 μg
$412
A01704-40
40 μg
$198
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit
Full Name THETM GFP Antibody, pAb, Rabbit
Abbreviated name-1
Green Fluorescent Protein antibody;
Documents
THE<sup>TM</sup> GFP Antibody, pAb, RabbitDocument-MSDS: 13472_20101124012427.PDF (PDF)
Figures
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
THE<sup>TM</sup> GFP Antibody, pAb, Rabbit zoomTHE<sup>TM</sup> GFP Antibody, pAb, Rabbit
Description
Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.
GenScript THETM GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.
Ig Subclass
IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript THETM GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
purified recombinant full-length GFP protein
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other Applications: user optimized
Conjugation
Unconjugated
Host Species
Rabbit
Note
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)
Document-COA
  • 10F000147_A01704_COA.pdf
  • Citation
    Kimberly J. Krager., et al. A Novel Biotinylated Lipid Raft Reporter For Electron Microscopic Imaging Of Plasma Membrane Microdomains. J Lipid Res. 2012 Oct;53(10):2214 - 2225.

    Kadauke S., et al. Tissue-specific mitotic bookmarking by hematopoietic transcription factor GATA1. Cell. 2012 Aug;150(4):725-37.

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