Catalog Products » Antibodies » antibody : Insulin Antibody (6E9F1), mAb, Mouse
Cat. No.
Name
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A01715-100
100 μg
$198
Insulin Antibody (6E9F1), mAb, Mouse
Full Name Insulin Antibody (6E9F1), mAb, Mouse
Abbreviated name-1
ILPR antibody;
Documents
Insulin Antibody (6E9F1), mAb, MouseDocument-MSDS: 13470_20101124012057.PDF (PDF)
Figures
Insulin Antibody (6E9F1), mAb, Mouse zoomInsulin Antibody (6E9F1), mAb, Mouse
Insulin Antibody (6E9F1), mAb, Mouse zoomInsulin Antibody (6E9F1), mAb, Mouse
Description
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Proinsulin is the prohormone precursor to insulin made in pancreas. It is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-Peptide. The C-Peptide is abstracted from the center of the proinsulin sequence; the two other ends (α and β chains) remain connected by disulfide bonds. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
GenScript Insulin Antibody (6E9F1), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant Insulin expressed in yeast.
Ig Subclass
IgG2a κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Insulin Antibody (6E9F1), mAb, Mouse detects human Insulin and slightly recognizes proinsulin, it has no cross-reactivity with C-peptide.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
human recombinant Insulin expressed in yeast
Clone ID
6E9F1
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/ml
ELISA Detection: 0.05-0.2 µg/ml
Recommended antibody pairing for sandwich immunoassay:

Other applications: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
Unconjugated
Species Reactivity
Human. Reactivity to other species is not tested yet.
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 10K000205_A01715_COA.pdf
  • 14468_20111114040022.PDF
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