Catalog Products » Antibodies » THE™ Elite Antibodies » THETM V5 Tag Antibody, mAb, Mouse
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Cat. No.
Product Name
Size
Price
Quantity
A01724-40 THETM V5 Tag Antibody, mAb, Mouse
40 μg
$119.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 μg
$395.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 μg
$219.00
THE<sup>TM</sup> V5 Tag Antibody, mAb, Mouse
Full Name
THETM V5 Tag Antibody, mAb, Mouse THE<sup>TM</sup> V5 Tag Antibody, mAb, Mouse
Abbreviated name-1
THETM V5 Tag Antibody, mAb, Mouse;
Documents
THE<sup>TM</sup> V5 Tag Antibody, mAb, MouseDocument-MSDS: A01724_MSDS.pdf (pdf)
Figures
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Figures
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Figures
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse zoomTHE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
Figures
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Figures
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Figures
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Figures
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse zoomTHE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
Figures
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse zoomTHE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
Description
The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Ig Subclass
IgG2a K
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen
V5 tag peptide conjugated to KLH
Clone ID
4C12E11
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
FACS: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Fusion Partner
Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Conjugation
Unconjugated
Host Species
Mouse
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)
Document-COA
  • 10L000228_A01724_COA
  • 15F000914_A01724_COA.pdf
  • Citation
    Liew CW, et al. Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic β cells. Proc Natl Acad Sci U S A. 2014June;111(22):E2319-28

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