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THETM V5 Tag Antibody, mAb, Mouse


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Cat. No.
Product Name
Size
Price
Quantity
A01724-40 THETM V5 Tag Antibody, mAb, Mouse
40 μg
$119.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 μg
$395.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 μg
$219.00



Abbreviated name-1 THETM V5 Tag Antibody, mAb, Mouse;
Description The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Immunogen V5 tag peptide conjugated to KLH
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Purification Method Protein A affinity column

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Clone ID 4C12E11
Ig Subclass IgG2a K
Specificity THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
FACS: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Comparison sensitivity of purified N-terminal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript (A01724, 0.2 µg/ml) and Invitrogen ( R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Sensitivity comparison of purified internal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript, (A01724, 0.2 µg/ml) and Invitrogen (R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Sensitivity comparison of purified internal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript, (A01724, 0.2 µg/ml) and Invitrogen (R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. The assay was performed with internal V5 tagged fusion protein.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Superior specificity assay of transfected cell lysates using Western blot with THE™ V5 Antibody, mAb, Mouse (GenScript, A01724).
Lane 1: V5-tagged protein transfected CHO cell lysate.
Lane 2: Non-transfected CHO cell lysate.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using THETM V5 Antibody, mAb, Mouse (GenScript, A01724). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Immunocytochemistry/Immunofluenrescence analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using THETM V5 Antibody, mAb, Mouse (GenScript, A01724). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using THETM V5 Antibody, mAb, Mouse (A01724, 1 µg/ml).
Lane 1: V5-tagged protein transfected HEK293 cell lysate as control.
Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with V5 Antibody, mAb, Mouse.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using
THETM V5 Tag Antibody, mAb, Mouse (GenScript, A01724) (black and red respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse


Cat. No.
Product Name
Size
Price
Quantity
A00623-100 V5-tag Antibody, pAb, Rabbit
100 μg
$199.00
A00877-40 V5-tag Antibody [HRP], pAb, Rabbit
40 μg
$99.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 μg
$219.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 μg
$395.00
A01733-100 THETM V5 Tag Antibody [HRP], mAb, Mouse
100 μg
$239.00
A01735-100 THETM V5 Tag Antibody [Biotin], mAb, Mouse
100 μg
$239.00
A00877-100 V5-tag Antibody [HRP], pAb, Rabbit
100 μg
$199.00
A01803-100 THETM V5 Tag Antibody [iFluor 488], mAb, Mouse
100 μg
$263.00
A01804-100 THETM V5 Tag Antibody [iFluor 555], mAb, Mouse
100 μg
$263.00
A01805-100 THETM V5 Tag Antibody [iFluor 647], mAb, Mouse
100 μg
$263.00
Liew CW, et al. Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic β cells. Proc Natl Acad Sci U S A. 2014June;111(22):E2319-28

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