Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
THETM NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/ml
Immunoprecipitation: 1-5 µg/ml
ICC/IF: 1-3 µg/ml
ELISA: 0.001-0.002 µg/ml
Flow cytometry: 1 µg/ml
Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007) The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Piuri M., et al. Generation of Affinity-Tagged Fluoromycobacteriophages by Mixed Assembly of Phage Capsids. Appl Environ Microbiol. 2013 Sep;79(18):5608-15.
Deqiang Duanmu., et al. Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival. Proc Natl Acad Sci U S A. 2013 Feb;110(9):3621-6.
Shanmugabalaji V., et al. Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants. Plant Mol Biol. 2013 Jan;81(1-2):13-25.
Marion Avril., et al. From The Cover: Pnas Plus: A Restricted Subset Of Var Genes Mediates Adherence Of Plasmodium Falciparum-Infected Erythrocytes To Brain Endothelial Cells. Proc Natl Acad Sci U S A. 2012 Jun;109(26):E1782 - E1790.
KolesiÅ„ski P., et al. Initial characteristics of RbcX proteins from Arabidopsis thaliana. Plant Mol Biol. 2011 Nov;77(4-5):447-459.
Paul Dominic B. Olinares., et al. Subunit Stoichiometry, Evolution, and Functional Implications of an Asymmetric Plant Plastid ClpP/R Protease Complex in Arabidopsis . Plant Cell. 2011 Jun;23(6):2348 - 2361.
Piotr Koprowski., et al. Genetic Screen for Potassium Leaky Small Mechanosensitive Channels (MscS) in Escherichia coli : RECOGNITION OF CYTOPLASMIC β DOMAIN AS A NEW GATING ELEMENT. J Biol Chem. 2011 Jan;286(1):877 - 888.
Yang W., et al. Development of a High-Throughput Screening Assay for Inhibitors of Small Ubiquitin-Like Modifier Proteases. J Biomol Screen. 2013 Jun;18(5):621 - 628 .