|Full Name || THETM NWSHPQFEK Tag Antibody,mAb,Mouse |
|THETM NWSHPQFEK Tag Antibody,mAb,Mouse || |
|Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins. |
|IgG1 Κ |
|The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
|THETM NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins. |
|0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
|NWSHPQFEK peptide conjugated to KLH |
|Protein A affinity column |
|Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. |
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
Western blot: 0.1-0.5 µg/ml
Immunoprecipitation: 1-5 µg/ml
ICC/IF: 1-3 µg/ml
ELISA: 0.001-0.002 µg/ml
Flow cytometry: 1 µg/ml
|Fusion of SP2/0-Ag14 myeloma and B-lymphocytes |
|ELISA Protocol (pdf)|
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)
| 11B000260_A01732_COA.pdf 13A000565_A01732_COA.pdf 14B000737_A01732_COA.pdf |
| Piuri M., et al. Generation of Affinity-Tagged Fluoromycobacteriophages by Mixed Assembly of Phage Capsids. Appl Environ Microbiol. 2013 Sep;79(18):5608-15. |
Deqiang Duanmu., et al. Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival. Proc Natl Acad Sci U S A. 2013 Feb;110(9):3621-6.
Shanmugabalaji V., et al. Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants. Plant Mol Biol. 2013 Jan;81(1-2):13-25.
Marion Avril., et al. From The Cover: Pnas Plus: A Restricted Subset Of Var Genes Mediates Adherence Of Plasmodium Falciparum-Infected Erythrocytes To Brain Endothelial Cells. Proc Natl Acad Sci U S A. 2012 Jun;109(26):E1782 - E1790.
KolesiÅ„ski P., et al. Initial characteristics of RbcX proteins from Arabidopsis thaliana. Plant Mol Biol. 2011 Nov;77(4-5):447-459.
Paul Dominic B. Olinares., et al. Subunit Stoichiometry, Evolution, and Functional Implications of an Asymmetric Plant Plastid ClpP/R Protease Complex in Arabidopsis . Plant Cell. 2011 Jun;23(6):2348 - 2361.
Piotr Koprowski., et al. Genetic Screen for Potassium Leaky Small Mechanosensitive Channels (MscS) in Escherichia coli : RECOGNITION OF CYTOPLASMIC β DOMAIN AS A NEW GATING ELEMENT. J Biol Chem. 2011 Jan;286(1):877 - 888.
Yang W., et al. Development of a High-Throughput Screening Assay for Inhibitors of Small Ubiquitin-Like Modifier Proteases. J Biomol Screen. 2013 Jun;18(5):621 - 628 .
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