p53 Antibody (2G5F7), mAb, Mouse

*This product has been discontinued!*

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. It is situated at the crossroads of signalling pathways that are essential for cell growth regulation and apoptosis. In normal unstressed cells, the level of p53 protein is downregulated via the binding of proteins such as MDM2, COP1, PIRH2 or JNK that promote p53 degradation via the ubiquitin/proteasome pathway. As most of these genes are up regulated by p53, this lead to a regulation loop that will keep p53 level very low in a normal cells. Alternative splicing of the human p53 gene gives rise of p53 beta, p53 gamma, Delta 40p53 (also known as p44, p47 or delat N p53), Delta 40p53 beta, Delta 40 p53 gamma, Delta 133p53, Delta 133p53 beta, and Delta 133p53 gamma.GenScript p53 Antibody (2G5F7), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant wild type p53 (wt p53).

Cat. No.	A01771
Size	Buy in bulk

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Overview Properties Background

Overview

Specificity	GenScript p53 Antibody (2G5F7), mAb, Mouse detects endogenous levels of p53 protein. Special reactivity with p53 isoforms of GenScript p53 antibody clones are tested by Western blot and described in the below table.
Host Species	Mouse
Immunogen	Human recombinant wild type p53
Species Reactivity	Human. Reactivity to other species is not tested yet.

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Properties

Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Purification	Protein A affinity column
Clone ID	2G5F7

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Background

Target Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. It is situated at the crossroads of signalling pathways that are essential for cell growth regulation and apoptosis. In normal unstressed cells, the level of p53 protein is downregulated via the binding of proteins such as MDM2, COP1, PIRH2 or JNK that promote p53 degradation via the ubiquitin/proteasome pathway. As most of these genes are up regulated by p53, this lead to a regulation loop that will keep p53 level very low in a normal cells. Alternative splicing of the human p53 gene gives rise of p53 beta, p53 gamma, Delta 40p53 (also known as p44, p47 or delat N p53), Delta 40p53 beta, Delta 40 p53 gamma, Delta 133p53, Delta 133p53 beta, and Delta 133p53 gamma.
GenScript p53 Antibody (2G5F7), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant wild type p53 (wt p53).

Synonyms

Tumor protein p53 mAb;

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