THETM Protein C Tag Antibody (HPC4), mAb, Mouse
|A01774-40||THETM Protein C Tag Antibody (HPC4), mAb, Mouse||
|A01774-100||THETM Protein C Tag Antibody (HPC4), mAb, Mouse||
|Abbreviated name-1||anti HPC4 Antibody, Protein C Antibody|
|Description||Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
GenScript THE™ Protein C (HPC4) Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.
|Immunogen||Protein C tag peptide conjugated to KLH|
|Purification Method||Protein A affinity column|
|Formulation||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript THE™ Protein C Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Protein C tagged fusion proteins.|
|Storage||GenScript THE™ Protein C Tag Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Flow cytometry: 1-3 µg for 1 x 106 cells.
ICC/IF: 1-3 µg/ml.
ELISA: 0.001-0.002 µg/ml.
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
IF & ICC Protocol(pdf)
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