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THETM Protein C Tag Antibody (HPC4), mAb, Mouse


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Cat. No.
Product Name
Size
Price
Quantity
A01774-40 THETM Protein C Tag Antibody (HPC4), mAb, Mouse
40 µg
$170.00
A01774-100 THETM Protein C Tag Antibody (HPC4), mAb, Mouse
100 µg
$350.00



Abbreviated name-1 anti HPC4 Antibody, Protein C Antibody
Description Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
GenScript THE™ Protein C (HPC4) Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.
Immunogen Protein C tag peptide conjugated to KLH
Host Species Mouse
Purification Method Protein A affinity column

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Clone ID 2H12F4
Ig Subclass IgG1,κ
Specificity GenScript THE™ Protein C Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Protein C tagged fusion proteins.
Storage GenScript THE™ Protein C Tag Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.1-0.5 µg/ml. Immunoprecipitation: 1-5 µg/ml. Flow cytometry: 1-3 µg for 1 x 106 cells. ICC/IF: 1-3 µg/ml. ELISA: 0.001-0.002 µg/ml.
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse
THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

Comparison of Protein C Tag Antibody, mAb, Mouse from (Left: GenScript A01774, 0.2 µg/ml) and Mouse Anti-Protein C mAb (Right: Roche, clone HPC4, 0.2 µg/ml) by Western blot. The assay was performed with purified N-terminal Protein C tagged fusion protein. The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse
THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

Comparison of Protein C Tag Antibody, mAb, Mouse from (Left: GenScript A01774, 0.2 µg/ml) and Mouse Anti-Protein C mAb (Right: Roche, clone HPC4, 0.2 µg/ml) by Western blot. The assay was performed with purified C-terminal Protein C tagged fusion protein. The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse
THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

Western blot analysis of immunoprecipitates from Protein C tagged protein transfected CHO cell lysates. Lane 1: Protein C tagged protein transfected CHO cell lysates as input control. Lane 2: Immunoprecipitates of the Protein C tagged protein transfected CHO cell lysates with THE™ Protein C Tag Antibody, mAb, Mouse (GenScript, A01774). Lane 3: Immunoprecipitates of the Protein C tagged protein transfected CHO cell lysates with Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007).

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse
THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of Protein C tagged protein transfected CHO cells
using THE™ Protein C Tag Antibody, mAb, Mouse (GenScript, A01774) and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007) The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse

THE<sup>TM</sup> Protein C Tag Antibody (HPC4), MAb, Mouse


Cat. No.
Product Name
Size
Price
Quantity
A00637-100 Protein C -Tag Antibody (HPC4), pAb, Rabbit
100 μg
$199.00
A00637-40 Protein C -Tag Antibody (HPC4), pAb, Rabbit
40 μg
$99.00


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