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THETM PEG Antibody, mAb, Mouse




Abbreviated name-1 THETM PEG Antibody, mAb, Mouse
Description PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalent couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs.PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Polyethylene glycol (PEG) antibody is a useful tool for the detection of PEGylated molecules.GenScript THETM PEG Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with PEG conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Method Affinity chromatography

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. For long-term storage, adding glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution is recommended.
Clone ID 5E10E9
Ig Subclass IgM
Specificity GenScript THETM PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.
Storage GenScript THETM PEG Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Note This product is not available in the US until Jan 1, 2017. Some reagents such as Tween-20, Triton X-100 and NP-40 interfere with the PEG antibody interaction due to structural similarity to PEG. Antibody solution should not contain such reagents in the assay of PEGylated molecules.

Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
ELISA Capturer: 5-10 µg/ml
ELISA Detector: 0.1-1.0 µg/ml
Competitive ELISA: customer optimized
Double Antigen Bridging ELISA: customer optimized
Western Blot: 0.1-1.0 µg/ml
Immunohistochemistry: 10-15 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

The half maximal inhibitory concentration (IC50) comparison of THETMPEG Antibody, mAb, Mouse (Cat.No. A01795-100,Clone 5E10E9) and the commercial Mouse Anti-PEG mAb (Clone AGP4) in competitive ELISA. The ELISA plate was coated by PEG40K-OVA, and unconjugated PEG40K was used for determining the IC50 of anti-PEG mAb. The test result showed GenScript PEG Antibody had lower IC50, therefore, better specificity and higher affinity to PEG were validated.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Double-antigen sandwich ELISA analysis of THETM PEG Antibody, mAb, Mouse (Cat.No. A01795-100) PEG Antibody was captured by PEG40K-OVA and detected by PEG20K-Biotin and Streptavidin-HRP conjugate. The test result showed GenScript PEG Antibody was suitable for Bridging ELISA application.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Sensitivity comparison of commercial Mouse Anti-PEG mAb (samples A,B: Clone AGP4, 0.2 ug/ml) with THETM PEG Antibody, mAb, Mouse (C,D: GenScript, A01795-100, 0.2 ug/ml) by Western Blot. A,C: PEGylated drug (Pegasys, Peginterferon Alfa 2A) B,D: Interferon Alfa 2A protein The test result showed GenScript PEG Antibody had better sensitivity and specificity than the other commercial product.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Sensitivity comparison of THETM PEG Antibody, mAb, Mouse (Cat.No. A01795-100,Clone 5E10E9) and commercial Mouse Anti-PEG mAb (Clone AGP4) by indirect ELISA, with plate coated with PEG40K-OVA. The test result showed GenScript PEG Antibody had better reactivity to PEG40K-OVA than the other commercial product.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Sensitivity comparison of THETM PEG Antibody, mAb, Mouse (Cat.No. A01795-100,Clone 5E10E9) and commercial Mouse Anti-PEG mAb (Clone AGP4) by indirect ELISA. The test result showed GenScript PEG Antibody had better reactivity to PEG20K than the other commercial product.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Sensitivity comparison of THETM PEG Antibody, mAb, Mouse (Cat.No. A01795-100,Clone 5E10E9) and commercial Mouse Anti-PEG mAb (Clone AGP4) by indirect ELISA. The test result showed GenScript PEG Antibody could detect PEG with different molecular weight and have better reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12) than the other commercial product.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

Immunohistochemistry analysis of mouse kidney tissue (Paraffin embedded) using THETM PEG Antibody, mAb, Mouse (A01795-100, 10 ug/ml) after mice were injected with BSA-PEG as an immunogen. The test result showed GenScript PEG Antibody was suitable for IHC application.

THE<sup>TM</sup> PEG Antibody, MAb, Mouse
THE<sup>TM</sup> PEG Antibody, MAb, Mouse

In-Cell ELISA analysis of PEGylated drug/HepG2 interaction using THETM PEG Antibody, mAb, Mouse (A01795-100, 1 ug/ml) after HepG2 cells were treated with PEGylated drug (Pegasys, Peginterferon Alfa 2A) at different concentrations. The test result showed GenScript PEG Antibody was suitable for the analysis of PEGylated drug/cell interaction.




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