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Peptide Design for Immunization Strategies Peptides created through GenScript's sophisticated design program have many advantages over full proteins when it comes to antibody production. Our Antigen Design Tool combines the industry's most advanced algorithms with GenScript's time-tested expertise. Each peptide antigen is measured against several protein databases to confirm the desired antibody and epitope specificity. GenScript stands behind its peptide design and carrier protein conjugation technology: We guarantee the delivery of effective antigens. Here are some of the factors that our total design program takes into account: 1. Length: Ideal antigen peptides have a length between 10 and 20 residues. Sequences of more than 20 residues increase the chance of covering an epitope but may also be susceptible to steric and conformational problems during peptide synthesis or conjugation. Sequences of 10 residues or fewer decrease the chance of covering a good epitope and may suffer from cross-reactivity due to potential homology over short sequences. 2. Antigenicity: The peptides that we design usually contain the long stretches of hydrophilic residues commonly found on the surface of native proteins and are therefore more likely to produce antibodies that will recognize the original protein of interest. 3. Stability: Unstable amino acids and sequences are either kept to a minimum or avoided. 4. Capping: Unless the customer requests otherwise, N-terminal residues are usually acetylated, and C-terminal residues are amidated. Peptide sequences from internal portions of their original proteins are capped on one terminus and conjugated on the other. This ensures that the peptide antigens do not show charges not present in the native protein. Such extra electrostatic charges may affect proper peptide folding and potentially alter antibody specificity. 5. Other considerations: Common sequence motifs such as the RGD motif, the helix-loop-helix sequence, GTP binding sites, and SH2 domains may cause cross-reactivity and should be avoided. Sequences associated with certain biological activities, such as autolytic cleavage sites, hormonal activity, and undesired post-translational modifications, are also avoided. Guaranteed Immune Response GenScript stands behind the promise of its antigen design system. For polyclonal antibody projects that use peptide antigens designed, synthesized, and conjugated by GenScript, we guarantee a final antibody product with an ELISA titer of 1:10,000 or better.
How to Order To request GenScript antigen design, please phone or email one of our service account managers, who will help you select the antibody service package best suited to your research needs.
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