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BacuVance™ baculovirus expression services
Baculovirus expression services quotation

BacuVance™ baculovirus expression

GenScript's BacuVance™ baculovirus expression platform was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells. As the largest gene synthesis supplier in the U.S., GenScript can provide you with free, advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of your protein.

There are several advantages of using baculovirus expression over E. coli such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins. For customers who prefer a eukaryotic expression system, are exploring lower-cost alternatives to mammalian expression but do not want to compromise on the overall quality of their recombinant protein, we strongly recommend considering our BacuVance™ baculovirus expression service. Using this baculovirus expression service we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. Alternatively, if you are looking for a fail-safe option for insect protein production at a competitive price, visit our InsectPower™ page to learn more.

Package details of BacuVance™ baculovirus expression services (SC1810)

Steps
Specifications Timeline# Deliverables Price

Gene synthesis and/or subcloning
  • Gene synthesis and codon optimization using our proprietary platforms (optional)
  • Subcloning into 1 or more expression vectors
2 weeks Construct(s) report  Baculovirus expression services quotation

Virus generation
  • Generation of recombinant Bacmid DNA
  • Transfection of insect cell with recombinant Bacmid DNA
  • Generation of P1 stock (low titer), P2 stock (high titer), and determination of virus titer by quantitative-PCR
  • Protein expression verification using anti-His or anti-GST antibody
2–3 weeks 5 ml, >107 pfu/ml virus stock

Pilot expression and purification evaluation
  • Infection of insect cells with P2 stock
  • 1-step affinity purification
  • SDS-PAGE gel and Western blot
1.5 weeks Expression data

1 L expression and purification
  • 1 L insect cell expression 
  • 1-step affinity purification
  • QC*
1–2 weeks Purified
proteins
QC* data

*QC: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and Western blot using anti-His or anti-GST antibodies will be performed, to determine protein identity. MALDI-TOF peptide mapping will be used in addition to Western Blot, to determine protein identity (if necessary)

#All timelines are approximate.

Package details of BacuVance™ baculovirus preparation services

GenScript's recombinant baculovirus preparation services provide guaranteed, consistent batch-to-batch virus. We provide titered, highly infectious virus stocks, ready-to-use along with detailed storage protocols.

Steps Specifications Timeline# Deliverables Catalog Price

Gene synthesis and/or subcloning
  • Gene synthesis and codon optimization using our proprietary platforms (optional)
  • Subcloning into expression vector
2 weeks Construct report    

Virus generation
  • Generation of recombinant Bacmid DNA
  • Transfection of insect cell with recombinant Bacmid DNA
  • Generation of P1 stock (low titer), P2 stock (high titer), and determination of virus titer by quantitative-PCR
  • Protein expression verification using anti-His or anti-GST antibody
 
2–3 weeks  Virus stock 10ml,
107 pfu/ml
SC1261 $1,900
Virus stock 100ml,
107 pfu/ml
SC1835 $2,250
Virus stock 100ml, 108 pfu/ml SC1840 $2,700
Virus stock, other volume and titer Baculovirus preparation services quotation

Baculovirus expression services quotation

Quotations and Ordering

Baculovirus expression services quotations & ordering

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