Blocking Peptides |
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It is distressingly common for antibodies to bind to proteins other than the intended antigen. However, these non-specific bindings can be identified. The band that is specific to immunizing peptide can be determined via immunizing peptide blocking experiment. GenScript provides blocking peptides as negative controls for antibody customers. These controls include affinity-purified rabbit and goat polyclonal antibodies and monoclonal antibodies raised against peptide antigens, including GenScript's celebrated custom peptide antigens. The binding of the antigen to the antibody may be reduced or blocked by pre-absorption with the blocking peptides.
GenScript offers over 400 blocking peptides for most of our phospho-specific and pan antibodies. These peptides are suitable to many applications, including Western blotting and immunohistochemistry assays. They can be used to confirm that the observed signal is due to the antibody’s interaction with target antigen and not to non-specific binding.
Click here to see more details about GenScript’s blocking peptides.
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