 Cell Apoptosis PI Detection Kit GenScript's Cell Apoptosis PI Detection Kit provides a rapid and convenient assay for apoptosis based upon fluorescent detection. The kit includes ready-to-use propidium iodide (PI), a DNA-specific red-fluorescence dye (excitation/emission maxima ≈ 535/617 nm). Propidium iodide is only permeant to dead cells. Under fluorescent microscopy, normal cells are not stained, while dead cells show strong red fluorescence. The intensity of the fluorescence of apoptotic cells is between dead cells and normal cells. Under flow cytometry, DNA in the cells is degraded by endogenous nuclease activity and diffused out of cells as part of apoptosis. A highly definable sub-G1 peak occurs and is easily quantified by PI. Cells can then be sorted and further analyzed by microscopy to verify the presence of apoptotic cells. The change in DNA of the apoptotic cells is then assayed by flow cytometry. | Key Features: - Extra Convenience: The kit is available in ready-to-use format.
- Simplified Procedure: Quick and easy to use.
- Strong Versatility: Directly analyze normal and apoptotic cells by flow cytometry, fluorescence microscopy, or fluorescent photometry.
| Examples:
Observe and analyze apoptosis by GenScript's Cell Apoptosis PI Detection Kit (L00311): P388 cells were induced apoptosis by 10 μM camptothecin for one hour at 37°C. The results are as follows: |
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