Immunochemistry is a preferred cell-based assay technology after a biochemical screening. However, the traditional immunochemical technologies, such as conventional Western, are cumbersome and labor intensive for compound screening. To address the problem, GenScript has developed Cellular Western assay service platform based on the In-Cell WesternTM technology.
The Cellular Western is an immunocytochemical assay performed in microplate format, 96-well or 384-well plate. Target-specific primary antibodies and infrared-labeled secondary antibodies are used to detect the change of target protein level in whole cell environment. Detection of the infrared fluorescence intensity enables quantitative analysis of cellular signaling pathway. Because of fewer steps and more real cellular environment than traditional Western technology, Cellular Western is a powerful tool which brings you higher throughput and more meaningful data in compound screening.