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Sequence in raw or FASTA format:

Database:

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Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.


Clone ID Definition Vector Stock Status Price *Turnaround time Order
OHu28570 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA. pcDNA3.1+-DYK On-demand $279.00 12-14
OHu28570C Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA. Customized vector On-demand $329.00 12-14

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Sequence Information ORF Nucleotide Sequence
Protein sequence
Vector pcDNA3.1+-DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+-DYK N terminal DYKDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
RefSeq Version NM_004333.4, 187608632
Length 2301 bp
Structure linear
Update Date 28-JUN-2014
Organism Homo sapiens (human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.
Product serine/threonine-protein kinase B-raf
Comment

Summary: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008].

RefSeq NP_004324.2
CDS 62..2362
Misc Feature(1)65..67
Misc Feature(2)65..67
Misc Feature(3)416..418
Misc Feature(4)500..502
Misc Feature(5)512..514
Misc Feature(6)527..742
Misc Feature(7)order(533..535,548..550,554..565,569..571,608..610,
Misc Feature(8)764..901
Misc Feature(9)order(764..766,803..805,812..814,842..844,851..853,
Misc Feature(10)order(785..787,794..799,824..829,830..832)
Misc Feature(11)order(794..796,800..802)
Misc Feature(12)1154..1156
Misc Feature(13)1154..1156
Misc Feature(14)1154..1156
Misc Feature(15)1178..1180
Misc Feature(16)1178..1180
Misc Feature(17)1199..1204
Misc Feature(18)1247..1249
Misc Feature(19)1256..1258
Misc Feature(20)1262..1264
Misc Feature(21)1262..1264
Misc Feature(22)1316..1318
Misc Feature(23)1346..1348
Misc Feature(24)1349..1351
Misc Feature(25)1373..1378
Misc Feature(26)1379..1381
Misc Feature(27)1397..1399
Misc Feature(28)1397..1399
Misc Feature(29)1397..1399
Misc Feature(30)1400..1402
Misc Feature(31)1430..2200
Misc Feature(32)1448..2206
Misc Feature(33)order(1448..1462,1472..1474,1502..1504,1508..1510,
Misc Feature(34)order(1448..1462,1472..1474,1502..1504,1508..1510,
Misc Feature(35)order(1460..1462,1667..1669,1673..1675,1787..1789,
Misc Feature(36)order(1838..1852,1865..1870,1910..1915)
Misc Feature(37)1856..1858
Misc Feature(38)1865..1867
Misc Feature(39)2072..2074
Misc Feature(40)2246..2248
Misc Feature(41)2246..2248
Misc Feature(42)2309..2311
Misc Feature(43)2318..2320
Exon (1)1..199
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (2)200..301
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (3)302..565
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (4)566..669
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (5)670..772
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (6)773..921
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (7)922..1041
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (8)1042..1201
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (9)1202..1238
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (10)1239..1375
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (11)1376..1493
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (12)1494..1578
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (13)1579..1755
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (14)1756..1802
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (15)1803..1921
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (16)1922..2053
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (17)2054..2188
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Exon (18)2189..2947
Gene:BRAF
Gene Synonym:B-RAF1; BRAF1; NS7; RAFB1
Translation MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEH IEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTV TSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRK TFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPI PQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQR DRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSP GPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHH LHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATV KSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNIN NRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARS LPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
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Position Chain Variation Link
43+dbSNP:
43+c, tdbSNP:71645935
44+c, gdbSNP:397507453
57+a, gdbSNP:71645936
97+a, gdbSNP:397507454
102+c, gdbSNP:397507455
125+a, gdbSNP:397507456
137+a, gdbSNP:397507457
complement(139)-c, adbSNP:371877084
150..161+, gcgccggcgccgdbSNP:397507458
153+c, gdbSNP:397516906
156..161+, gcgccgdbSNP:397507459
161..162+, gcgccgdbSNP:397515331
complement(236)-dbSNP:
complement(236)-t, cdbSNP:371504803
complement(340)-dbSNP:
complement(340)-t, gdbSNP:150050723
complement(349)-t, cdbSNP:373545899
386+a, tdbSNP:397507460
complement(406)-t, cdbSNP:149547328
411+g, tdbSNP:397507461
426+a, cdbSNP:397507462
complement(427)-g, adbSNP:201123489
complement(436)-c, adbSNP:201507202
460+a, gdbSNP:397507463
complement(467)-c, adbSNP:138086018
complement(490)-t, adbSNP:370069295
500+a, gdbSNP:397507464
complement(544)-g, cdbSNP:61730029
complement(562..563)-, tdbSNP:35546910
complement(640)-dbSNP:
complement(640)-t, cdbSNP:142723570
complement(769)-dbSNP:
complement(769)-g, adbSNP:138333692
complement(773)-dbSNP:
complement(773)-t, cdbSNP:373935661
775+a, tdbSNP:397516902
782+a, cdbSNP:387906661
783+a, c, g, tdbSNP:387906660
complement(784)-t, cdbSNP:369182143
791+a, cdbSNP:397507465
796+a, c, tdbSNP:397507466
797+c, gdbSNP:180177034
800+g, tdbSNP:397516903
801+c, tdbSNP:397507467
802+g, tdbSNP:397509343
814+c, tdbSNP:397507468
830+a, cdbSNP:397507469
831+a, gdbSNP:180177035
845+a, cdbSNP:397507470
846+a, cdbSNP:397516904
854+c, gdbSNP:397516905
complement(889)-g, adbSNP:199790021
complement(916)-t, cdbSNP:376575252
complement(959)-dbSNP:
complement(959)-t, cdbSNP:372239545
complement(962)-g, adbSNP:34776339
complement(976)-t, cdbSNP:145675911
1029+c, tdbSNP:397516907
complement(1038)-g, adbSNP:368435578
complement(1053)-dbSNP:
complement(1053)-g, adbSNP:372915332
complement(1070)-g, adbSNP:200373902
complement(1081)-t, adbSNP:369837503
complement(1084)-t, cdbSNP:56092510
complement(1085)-t, cdbSNP:201481342
complement(1090)-t, cdbSNP:140486841
complement(1093)-t, cdbSNP:56094800
1101+c, gdbSNP:397516884
complement(1109)-c, adbSNP:202209106
complement(1116)-t, cdbSNP:201882896
complement(1127)-g, cdbSNP:151308995
complement(1129)-t, cdbSNP:143335467
1145+a, cdbSNP:397516885
complement(1161)-g, adbSNP:267601317
complement(1168)-g, cdbSNP:375568418
1181+a, gdbSNP:397507471
complement(1205)-dbSNP:
complement(1205)-t, adbSNP:147732750
1226+c, tdbSNP:397507472
1244+dbSNP:
1244+a, gdbSNP:397516886
complement(1264)-g, adbSNP:370130654
complement(1266)-t, gdbSNP:199927105
complement(1267)-t, gdbSNP:201758035
1270+c, tdbSNP:397516887
complement(1288)-t, cdbSNP:145035762
1297+c, tdbSNP:397516888
complement(1298)-t, cdbSNP:377093637
1391+dbSNP:
1391+c, tdbSNP:121913371
complement(1393)-t, cdbSNP:56101602
1408+c, tdbSNP:397516889
complement(1444)-t, cdbSNP:56216404
1446+g, tdbSNP:180177032
1449+g, tdbSNP:180177033
1451+c, gdbSNP:121913349
1452+a, c, g, tdbSNP:121913348
1457+a, c, gdbSNP:121913353
1458+a, c, g, tdbSNP:121913351
1464+c, tdbSNP:397507473
1466..1468+agc, agt, ggadbSNP:121913358
1466..1468+, ggadbSNP:397516890
1466+a, c, gdbSNP:121913357
1467+a, c, g, tdbSNP:121913355
1470+c, gdbSNP:397516891
1472+a, g, tdbSNP:121913376
1503+dbSNP:
1503+a, cdbSNP:397516892
1508+a, cdbSNP:397507474
1515+c, tdbSNP:397507475
1516+c, gdbSNP:180177036
1521+g, tdbSNP:397516893
complement(1529)-g, adbSNP:375520366
1556+a, gdbSNP:180177037
1558+a, c, tdbSNP:397507476
1562+a, c, gdbSNP:180177038
1563+a, c, g, tdbSNP:180177039
1574+c, tdbSNP:397507477
1653+dbSNP:
1653+g, tdbSNP:397507478
1656+a, gdbSNP:397507479
1661+c, gdbSNP:180177041
complement(1678)-g, adbSNP:371497168
complement(1693)-g, adbSNP:149702741
1756+dbSNP:
1756+g, tdbSNP:397507480
complement(1765)-g, adbSNP:199714538
1781+c, tdbSNP:397516894
1783+c, gdbSNP:397507481
complement(1786)-t, cdbSNP:200228275
1802+a, c, gdbSNP:180177040
1803+dbSNP:
1803+a, gdbSNP:121913370
1804+a, tdbSNP:397516895
1810+g, tdbSNP:397507482
1817+a, gdbSNP:121913340
1821+a, cdbSNP:121913335
1822+a, cdbSNP:121913336
1835+a, gdbSNP:121913363
complement(1836)-t, g, adbSNP:55939351
1837+a, gdbSNP:121913362
1841+a, gdbSNP:397516896
1842+a, g, tdbSNP:121913338
1843+a, tdbSNP:121913337
1845+c, tdbSNP:121913225
1846+g, tdbSNP:121913341
1847+c, g, tdbSNP:121913361
1848+g, tdbSNP:397507483
1850..1851+ct, tcdbSNP:121913368
1850+c, g, tdbSNP:121913369
1851+a, g, tdbSNP:121913366
1857+c, tdbSNP:121913375
1858+a, tactacgdbSNP:121913374
1859..1860+aa, ag, gtdbSNP:121913227
1859+a, g, tdbSNP:121913378
1860..1862+, tgadbSNP:397516897
1860..1861+at, tgdbSNP:121913377
1860+a, c, g, tdbSNP:113488022
1862..1864+, aaadbSNP:121913226
1862+a, c, gdbSNP:121913364
1863+a, c, tdbSNP:397507484
1864+a, cdbSNP:121913365
1867+c, tdbSNP:191566526
1868+c, tdbSNP:104886015
complement(1869)-t, cdbSNP:372569965
1874..1875+ag, ttdbSNP:121913372
1875+a, gdbSNP:121913373
1975+dbSNP:
1975+a, tdbSNP:180177042
complement(1990)-t, cdbSNP:9648696
complement(2071)-dbSNP:
complement(2071)-t, adbSNP:201793951
2073+a, gdbSNP:397507485
complement(2136)-g, adbSNP:55715359
complement(2143)-t, cdbSNP:375174370
2187+a, gdbSNP:397507486
complement(2197)-dbSNP:
complement(2197)-g, adbSNP:377165711
2216+c, tdbSNP:397507487
complement(2217)-c, adbSNP:368528867
complement(2257)-g, cdbSNP:142592480
2290+c, tdbSNP:397516901
complement(2296)-t, cdbSNP:56046546
complement(2307)-t, gdbSNP:200490285
complement(2352)-g, adbSNP:139420557
complement(2367)-t, cdbSNP:200360246
complement(2388)-c, adbSNP:202148822
complement(2419)-t, adbSNP:143647707
complement(2429)-t, cdbSNP:373128505
complement(2455..2456)-, adbSNP:200393520
complement(2577)-t, adbSNP:140083479
complement(2578)-g, adbSNP:184804021
complement(2737)-t, cdbSNP:192498662
complement(2749)-t, cdbSNP:114105685
Gene SymbolBRAF
Gene SynonymB-RAF1; BRAF1; NS7; RAFB1
Chromosome7
Locus Map7q34
Title Copper is required for oncogenic BRAF signalling and tumorigenesis .
Author Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ and Counter CM.
Journal Nature 509 (7501), 492-496 (2014)
Title BRAF genetic heterogeneity in papillary thyroid carcinoma and its metastasis .
Author Walts AE, Pao A, Sacks W and Bose S.
Journal Hum. Pathol. 45 (5), 935-941 (2014)
Title BRAF pyrosequencing analysis aided by a lookup table .
Author Olson MT, Harrington C, Beierl K, Chen G, Thiess M, O'Neill A, Taube JM, Zeiger MA, Lin MT and Eshleman JR.
Journal Am. J. Clin. Pathol. 141 (5), 639-647 (2014)
Title Costello Syndrome .
Author Gripp,K.W. and Lin,A.E.
Journal (in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Title LEOPARD Syndrome .
Author Gelb,B.D. and Tartaglia,M.
Journal (in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Title Cardiofaciocutaneous Syndrome .
Author Rauen,K.A.
Journal (in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Title Noonan Syndrome .
Author Allanson,J.E. and Roberts,A.E.
Journal (in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Title 95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site .
Author Stephens RM, Sithanandam G, Copeland TD, Kaplan DR, Rapp UR and Morrison DK.
Journal Mol. Cell. Biol. 12 (9), 3733-3742 (1992)
Title Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94Braf/Rmil and B-raf-2, a processed pseudogene .
Author Eychene A, Barnier JV, Apiou F, Dutrillaux B and Calothy G.
Journal Oncogene 7 (8), 1657-1660 (1992)
Title B-raf and a B-raf pseudogene are located on 7q in man .
Author Sithanandam G, Druck T, Cannizzaro LA, Leuzzi G, Huebner K and Rapp UR.
Journal Oncogene 7 (4), 795-799 (1992)

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