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GenScript Oligo Calculation Tool
Calculation Methods:
Molecular Weight (Free Acid) = #A x 313.2 + #C x 289.2 + #G x 329.2 + #T x 304.2 + #U x 290.2 + #I x 252 - 62
Millimolar Extinction Coefficient (OD/�mol) = #A x 15.3 + #C x 7.4 + #G x 11.8 + #T x 9.3 + #U x 10.1 + #I x 12.25
µg/OD = Molecular Weight (Free Acid) / Extinction Coefficient
Tm* = (Enthalpy (H) / (Entropy (S) + Molar Gas Constant x ln(C/4)) + 16.6 log [k+] + 273.15
*Breslauer KJ, Frank R, Blocker H, Marky LA. Proc. Natl. Acad. Sci. USA, 83:3746-3750.
Millimolar Extinction Coefficient (OD/�mol) = #A x 15.3 + #C x 7.4 + #G x 11.8 + #T x 9.3 + #U x 10.1 + #I x 12.25
µg/OD = Molecular Weight (Free Acid) / Extinction Coefficient
Tm* = (Enthalpy (H) / (Entropy (S) + Molar Gas Constant x ln(C/4)) + 16.6 log [k+] + 273.15
*Breslauer KJ, Frank R, Blocker H, Marky LA. Proc. Natl. Acad. Sci. USA, 83:3746-3750.