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WST-1

*This product has been discontinued!*
The tetrazolium salt WST-1 is cleaved to formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is mostly dependent on the glycolytic NAD(P)H production of viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture. The stability of WST-1 allows for its formulation as a ready-to-use solution.Suitable for cell proliferation and cytotoxicity assays.
C01672
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Description

The tetrazolium salt WST-1 is cleaved to formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is mostly dependent on the glycolytic NAD(P)H production of viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture. The stability of WST-1 allows for its formulation as a ready-to-use solution.Suitable for cell proliferation and cytotoxicity assays.
Synonyms

4-[3-(4-Idophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate

Product Introduction
Cas No 150849-52-8
Appearance Pale yellowish brown or pale brown powder
Color Pale
Formazan 438 nm
Heavy Metals ≤3ppm
Moisture ≤5%
Molar abso min.21,600 (244 nm)；37,000(formazan)
Molecular Formula C19H11IN5O8S2Na
Molecular Weight 651.35
Solubility 100 mg/10 ml water, 6.5 mg/10 ml 50 mM Tris buffer, pH 8.0
Tlc analysis Pass test
Storage 0-5 ºC
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