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CloneEZ® PCR Cloning Kit

GenScript'sproprietary CloneEZ^® Seamless cloning technology is a cutting-edge, recombination-based cloning technology that can accurately and efficiently assemble genes and gene fragments in any combination of sequences. The CloneEZ^® PCR Cloning Kit is designed for the quick cloning of PCR products without any headaches of restriction sites. The universal CloneEZ^® cloning method works with any insert and any vector at any restriction site. Using our proprietary CloneEZ^® enzyme, this kit rapidly generates precise, directional constructs in 30-min at a room temperature.

CloneEZ^® PCR Cloning Kit can be used for all types of cloning that involves the joining of DNA fragments and also has a variety of applications:

PCR cloning of up to 12 kb
High-throughput (HTP) PCR cloning
Gene transfer from one vector to another
In vitro joining of DNA fragments

Key Features:

Fast and Easy: 30-minute, one-step incubation at room temperature, seamless joints with no extra or unwanted base pairs
High Efficiently: Success rate over 95%
Universal: Clone any insert into any location, within any vector you choose
Compatibility: The kit is highly compatible with high-throughput PCR cloning

Products Details
FAQs
Examples

CloneEZ^® PCR Cloning Kit can cut down PCR Cloning procedure to 30 min from traditional 3.5 hrs, with one-step procedure instead of multiple steps.

	CloneEZ® Seamless Technology			Classical Cloning
Directional	√			Restriction digestion Gel extraction Ligation
Restriction enzymes not required	√			X
Ligase not required	√			X
Reaction Time	0.5 hr			>3.5 hr
Reagents Included	CloneEZ^® enzyme (50 ul, 5 U/ul) 10X Buffer (100 ul) pUC57 Linear Vector (Kpnl/HindIII, 10 ul, 100 ng/ul) 1 kb contract insert ( 10 ul, 100 ng/ul)			Ligase enzyme 10X Buffer
High-efficiency cloning with long fragments (15-20 bp recommend)	4 kb	2 kb	1 kb	NA
	93%	95%	100%	NA

Components	L00339
CloneEZ^® Seamless Enzyme (5 U/μl)	50 μl
10X CloneEZ^® Seamless Buffer	100 μl
pUC57 Linearized (KpnI/HindIII,100 ng/μl)	10 μl
1-kb Control Insert (100 ng/μl)	10 μl

Ordering Information

	Cat.No.	Name	Size	Price	Technical Manual	Quatity
	L00339	CloneEZ^® PCR Cloning Kit	24 rxns	$175.00

General Questions What are the differences between the CloneEZ^® kit and the PCR Cloning Kit? The technical information in patent document is not available in the manual, why is that?
Transformation Loading here... Why don't I get sufficient colonies when the CloneEZ^® reaction is transformed? Why do I get incorrect colonies when the CloneEZ^® reaction is transformed?
Primer Design Loading here... What factors need to be considered when designing the primer? To be compatible for CloneEZ^® cloning method, what should the purity of my primer? Can multiple fragments be cloned into a single vector using this kit? Can I use CloneEZ^® PCR Cloning Kit with my own vectors? What factors need to be considered when using CloneEZ^® PCR Cloning Kit with customers' vector? Will the CloneEZ^® Cloning reaction work more efficiently if I use primers that contain a longer than 15 base region of homology? How is the stability of the enzyme included in CloneEZ^® PCR Cloning Kit? Loading FAQs

A: Shown below is number and percentage of positive clones obtained with the CloneEZ^® Kit.

	1 kb	2 kb	4 kb
Number of positive clones	~1200	~800	221
Recombinant rate (%)	100%	95%	93%

B: Shown below is PCR screening result of positive clones obtained with the CloneEZ^® Kit.
PCR cloning kit