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CloneEZ PCR Cloning Kit

CloneEZ® PCR Cloning Kit

Traditional cloning is a tedious and time-consuming procedure. The numerous intermediate steps and subcloning into the destination vector can clog up valuable lab time and resources. GenScript's CloneEZ® Seamless cloning technology provides a swift and reliable alternative to PCR cloning. It is particularly useful for high-throughput (HTP) PCR cloning. The GenScript CloneEZ® Kit can directly fuse PCR products of up to 12 kb into any destination vector without the use of restriction enzymes or ligases, bypassing the intermediate steps of the cloning process. GenScript's proprietary CloneEZ® Seamless Enzyme, included in the kit, can fuse your PCR product into the destination vector at a very high level of efficiency, completing the process in only 30 minutes when performed at room temperature (20°C - 25°C). Our CloneEZ® Kit can be used for all types of cloning that involves the joining of DNA fragments.

Key Features:

  • Fast and simple procedure: The kit requires only one 30-minute incubation at room temperature.
  • High efficiency: Recombination efficiency is above 95%.
  • Large fragments: This product works with any DNA sequence of up to 12 kb.
  • Compatibility: The kit is highly compatible with high-throughput PCR cloning.
  • Components
  • CloneEZ® Seamless Technology
  • Example
Cat. No.
Name
Size
Price
Quatity
Technical Manual
L00339 CloneEZ® Kit 24 rxns $175.00 CloneEZ Kit

Components
L00339
CloneEZ® Seamless Enzyme (5 U/μl)
50 μl
10X CloneEZ® Seamless Buffer
100 μl
pUC57 Linearized (KpnI/HindIII,100 ng/μl)
10 μl
1-kb Control Insert (100 ng/μl)
10 μl
CloneEZ Kit

GenScript’s recently developed CloneEZ® Seamless cloning technology offers high efficiency, quick and direct cloning, bypasses the tedious procedures of traditional cloning that often involve restriction, ligation, and sometimes phosphorylation. This technology works with any DNA sequence and any vector. The only requirement is that your PCR primers have to contain 15 or more nucleotide-long sequence homologous to vector sequence. The diagram shows the procedure of CloneEZ® Seamless cloning.

CloneEZ Kit
Click to enlarge
CloneEZ Kit
Figure 1: CloneEZ® Seamless cloning can cut down PCR cloning procedure to 30 min from traditional 3.5 hours, with one-step procedure instead of multiple steps.