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OptimumGeneTM - Codon Optimization



Minimum 10-fold increase in protein express level
  

As one of the world's leading suppliers of gene synthesis services, GenScript has a proven record of dedication to innovation in our constant efforts to integrate new and updated technology into our gene expression repertoire. GenScript’s OptimumGeneTM Gene Design system is a proprietarily gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system. The OptimumGeneTM algorithm takes into consideration of a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation.

codon optimization
  • Advantages
  • Algorithm
  • Track Record
  • Case Study

Advantages

Minimum 10-fold increase in protein express level
  

Fruitful results in even the most difficult sequences: GenScript's painstakingly crafted algorithms can optimize even highly repeated sequences, extremely long sequences, and sequences with large numbers of parameters. Our personal best is over 30, and we're willing to challenge it any time.

Comprehensive usage tables for optimization in any host: GenScript boasts an extensive collection of proprietary codon usage tables, allowing you to use any host, any time in either basic research or industrial applications. Specifically, we have dependable codon tables for organisms, such as Pichia and Sf9, which are widely used in development procedures.

DNA vaccine design: GenScript provides an effective solution to your vaccine needs, screening and optimizing parameters involved in regulating vaccinal gene expression and immunogenicity.

Customized solutions: The optimization conditions can be tailored to meet the specific needs of even the most esoteric project.

Related Resources

Use FREE Rare Codon Analysis Tool to check if your gene needs to be optimized.

Optimization Parameters

OptimumGeneTM algorithm optimizes a variety of parameters that are critical to transcription, translation and protein folding:

Transcriptional Efficacy:
  • GC content
  • CpG dinucleotides content
  • Cryptic splicing sites
  • Negative CpG islands
  • SD sequence
  • TATA boxes
  • Terminal signal
    		•
    Translation Efficiency:
    • Codon usage bias
    • GC content
    • mRNA secondary structure
    • Premature PolyA sites
  • RNA instability motif (ARE)
  • Stable free energy of mRNA
  • Internal chi sites and ribosomal binding sites
    		•
    Protein Refolding:
    • Codon usage bias
    • Interaction of codon and anti-codon
  • Codon-context
  • RNA secondary structures
    		•

    Selection Volume

    OptimumGeneTM produces over 10,000 candidate sequences during round one alone. A complex sorting matrix then selects for the best sequence for each specific customized request.

    codon optimization

    OptimumGeneTM History of Successful Codon Optimization

    GenScript has accumulated years of experience in codon optimization and gene synthesis. Our codon optimization system has optimized over 50,000 sequences in almost all major expression systems. Many top pharmaceutical companies are our customers.

    Customer Testimonial

    "I think GenScript provides absolutely excellent products in a timely fashion. I have primarily used genscript to order codon optimized artificial genes. Ordering artificial genes on their website or via Email makes this especially facile because I can simply inquire about a gene, get a quote and then get a PO#. The whole process is fast and the turnaround time for obtaining the finished product is usually less than a month. I also think their technical support are wonderful people, they have been most helpful in facilitating the process. I am so impressed with them that I have recommended them to several of my colleagues."

    ― Dr. Maria Schumacher, The University of Texas. M.D. Anderson Cancer Center

    Publications

    Ning Shao and Ralph Bock. A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii. Curr Genet. Jun 2008
    Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. 6. Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study. Protein Expr Purif. May 2008; 59(1): 94-102
    V. Melkebeek, E. Sonck, F. Verdonck, B. M. Goddeeris, and E. Cox. Optimized FaeG Expression and a Thermolabile Enterotoxin DNA Adjuvant Enhance Priming of an Intestinal Immune Response by an FaeG DNA Vaccine in Pigs. Clinical and Vaccine Immunology. Jan 2007; 14(1): 28-35

    Case Study

    Minimum 10-fold increase in protein expression level in E.coli

    Figures below manifested the effectiveness of the OptimumGeneTM technology in increasing protein expression levels for two different genes expressed in E.coli.

     

    Fig. A. GenScript's OptimumGeneTM codon optimization delivered 10 times higher expression level for protein α compared to non-optimized native gene sequence. The expression level was 3 times more than that from Competitor GA's optimization method.
     

    Fig. B. GenScript's OptimumGeneTM codon optimization delivered 20 times higher expression level for protein β compared to non-optimized native gene sequence. The expression level was 13 times more than that from Competitor GA's optimization method.
    Note: GenScript will not claim any rights whatsoever over any intellectual property or technical protocols involved in OptimumGeneTM codon optimization and gene synthesis services such as gene sequences or any materials submitted by the customer. GenScript will not release such information to any third party without customer consent. Sensitive information is only disclosed to the personnel responsible for the successful operation of the project on a need-to-know basis. GenScript will sign a non-disclosure or confidentiality agreement to ensure customers’ full rights over IP for the optimized and synthesized genes if needed.