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plasmid DNA preparation

Plasmid DNA Preparation Services

GenScript plasmid DNA preparation services are amenable to small research labs as well as large-scale manufacturing biotech and pharmaceutical companies. Our high-quality plasmids will help you achieve highly efficient cell transfection, as well as support DNA vaccine development, antibody production, and other preclinical research projects.

Our plasmid DNA preparation platform has recently been upgraded to provide high-quality plasmid preparation at competitive prices. Choose from our Research Grade and Transfection Grade packages to obtain the purified plasmid that best fits your research needs. Purification for both high-copy and low-copy plasmids, as well as animal-free processing, is available.

New! High-Throughput (HT) Plasmid Prep service: Now available through GenScript, our HT Plasmid Prep service will allow you to further customize your research needs through GenScript. Available in 10 ug and 30 ug quantities with no minimum order number, this new service will allow you to order more preps at a lower price! Find out more here.

Plasmid Prep Service Advantages


  Research Grade Transfection Grade
Animal-free cultivation media Included Included
Advanced animal-free prep1 Optional Optional
Supercoiling Predominantly supercoiled ≥ 90% supercoiled2
Endotoxin levelWhat's this? - ≤ 0.01 EU/µg3
Bioburden assay analysis Optional Included
Sterile filtering Optional Optional
Deliverables µg to g amounts of plasmid according to your specifications
Turnaround time 5 business days for < 2 mg 5 business days for < 500 μg
QC Items Appearance
A260/280
Supercoiled content
Residual RNA
Genomic DNA
Concentrations
Restriction Analysis
Endotoxin Assay (LAL test) -
Bio-burden Assay Optional

1 Both Research and Transfection Grade preps are prepared using cultivation media without any animal-derived components. For clinical applications and animal studies, the advanced animal-free prep can be included for additional control over potential safety risks. See our FAQ for more details.
2 Our standard supercoiled content is measured analytically by densitometry, of which the data obtained is for information only.
3 We can provide more stringent QC if desired. Please indicate your prep requirements on the quote form or contact us for more information.

Quality Control

GenScript's plasmid DNA preparation services offer the most aggressive quality control standards on the market, and services can be customized to your quality and quantity needs. Preps are offered at up to ≥ 90% supercoil and ≤ 0.01 EU/µg endotoxin levels.

QC items Specification Analytical Method
Appearance
Clear, no visible particles
Visual inspection
A260/280
1.8-2.0
Nanodrop UV absorption
Concentration
On request, default 1 mg/ml
UV absorption
Supercoil content
For information only
Densitometry upon agarose gel electrophoresis
Residue RNA
Not visible
Visual inspection upon electrophoresis
Genomic DNA
Not visible
Visual inspection upon electrophoresis
Restriction Analysis
Conforms to reference
Enzyme digestion and electrophoresis
Endotoxin assay
Based on grade
LAL assay
Bioburden assay
No growth on agar plate after 48 hours
Bioburden

* Sterile filtering and animal-free processing is available at an additional charge; click here to request a quote.
For more information about our QC measures, click here.

Common applications for plasmid DNA preparation

Plasmid DNA prep is a useful tool for many downstream applications

Research Grade
  • Molecular cloning
  • Sequencing
  • Mutagenesis
  • Southern blot
  • Library construction
  • Probe synthesis
  • Transfection or transformation (recommended for E. coli, bacillus, mold, yeast)
Transfection Grade
  • Highly effective transfection and co-transfection (recommended for mammalian cells)
  • Antibody production
  • Gene vaccine and gene therapy studies
  • Protein manufacturing
  • Animal studies

Frequently Asked Questions

Q: What is "animal-free cultivation media"? What is the advanced animal-free prep process and when would I need it?
A: For our research and transfection grade plasmid prep services, we are using cultivation media without any animal-derived components, making the media "animal-free."
Unlike traditional plasmid prep that uses bovine pancreatic RNase, our animal-free prep process uses a recombinant RNase which is guaranteed bovine-free. The advanced animal-free prep process is critical for clinical applications and animal studies to avoid safety risks.

Q: Why measure the supercoiled percent?
A: Multiple studies have shown that highly supercoiled plasmids have many applications for both in vivo and in vitro work. For example, supercoiling can strongly influence transfection efficiency, and can have several sides effects for DNA therapy. In addition, the supercoil percent has been determined as a predictor of plasmid performance and is recognized by many regulatory groups.

Q: How do endotoxins affect my research goals?
A: Endotoxins can have many adverse downstream effects on your research projects, ranging from decreased transformation efficiencies to adverse side-effects in animal studies. To learn more about endotoxins and other QC details, click here.

Customer Testimonial

"We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!"

― Dr. Melissa A. Melan, University of Pittsburgh Medical Center, USA

Selected Publications

  • Glenn K, Smith KS. Allosteric Regulation of Lactobacillus plantarum Xylulose 5-Phosphate/Fructose 6-Phosphate Phosphoketolase (Xfp). J Bacteriol. 2015 Apr;197(7);1157-63
  • Petrillo C, Cesana D, Piras F, et al. Cyclosporin a and rapamycin relieve distinct lentiviral restriction blocks in hematopoietic stem and progenitor cells. Mol Ther. 2015 Feb;23(2);352-62
  • Simons C, Rash LD, Crawford J, et al. Mutations in the voltage-gated potassium channel gene KCNH1 cause Temple-Baraitser syndrome and epilepsy. Nat Genet. 2014 Dec;47(1);73-7
  • See more recent peer-reviewed publications that cite GenScript's Plasmid Prep. Services


Delivery specifications and ordering information

When requesting a quote for the plasmid DNA preparation services, please specify:

Quotations and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.

 
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