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plasmid DNA preparation

Plasmid DNA Preparation Services

GenScript plasmid DNA preparation services are amenable to small research labs as well as large-scale manufacturing biotech and pharmaceutical companies. Our high-quality plasmids will help you achieve highly efficient cell transfection, as well as support DNA vaccine development, antibody production, and other preclinical research projects.

Our plasmid DNA preparation platform has recently been upgraded to provide high-quality plasmid preparation at competitive prices. Choose from our Research Grade and Transfection Grade packages to obtain the purified plasmid that best fits your research needs. Purification for both high-copy and low-copy plasmids, as well as animal-free processing, is available.

New! High-Throughput (HT) Plasmid Prep service: Now available through GenScript, our HT Plasmid Prep service will allow you to further customize your research needs through GenScript. Available in 10 ug and 30 ug quantities with no minimum order number, this new service will allow you to order more preps at a lower price! Find out more here.

Plasmid Prep Service Advantages

  • Economical: 100 µg miniprep starting from just $50
  • Flexible: Extensive quantity options
  • Quality Assured: Minimum of 7 stringent QC parameters analyzed for each step
  • Fast turnaround time: Delivery from 5 days, only an additional 2 days with any gene synthesis order
  • One-stop downstream solutions: combine with gene synthesis, custom cloning, or mutagenesis

  • Service Specifications
  • Quality Control
  • Applications
  • Frequently Asked Questions
  • Customer Testimonial
  Research Grade Transfection Grade
Animal-free cultivation media Included Included
Advanced animal-free prep1 Optional Optional
Supercoiling Predominantly supercoiled ≥ 90% supercoiled2
Endotoxin levelWhat's this? - ≤ 0.01 EU/µg3
Bioburden assay analysis Optional Included
Sterile filtering Optional Optional
Deliverables µg to g amounts of plasmid according to your specifications
Turnaround time 5 business days for < 2 mg 5 business days for < 500 μg
QC Items Appearance
A260/280
Supercoiled content
Residual RNA
Genomic DNA
Concentrations
Restriction Analysis
Endotoxin Assay (LAL test) -
Bio-burden Assay Optional

1 Both Research and Transfection Grade preps are prepared using cultivation media without any animal-derived components. For clinical applications and animal studies, the advanced animal-free prep can be included for additional control over potential safety risks. See our FAQ for more details.
2 Our standard supercoiled content is measured analytically by densitometry, of which the data obtained is for information only.
3 We can provide more stringent QC if desired. Please indicate your prep requirements on the quote form or contact us for more information.

Quality Control

GenScript's plasmid DNA preparation services offer the most aggressive quality control standards on the market, and services can be customized to your quality and quantity needs. Preps are offered at up to ≥ 90% supercoil and ≤ 0.01 EU/µg endotoxin levels.

QC items Specification Analytical Method
Appearance
Clear, no visible particles
Visual inspection
A260/280
1.8-2.0
Nanodrop UV absorption
Concentration
On request, default 1 mg/ml
UV absorption
Supercoil content
For information only
Densitometry upon agarose gel electrophoresis
Residue RNA
Not visible
Visual inspection upon electrophoresis
Genomic DNA
Not visible
Visual inspection upon electrophoresis
Restriction Analysis
Conforms to reference
Enzyme digestion and electrophoresis
Endotoxin assay
Based on grade
LAL assay
Bioburden assay
No growth on agar plate after 48 hours
Bioburden

* Sterile filtering and animal-free processing is available at an additional charge; click here to request a quote.
For more information about our QC measures, click here.

Delivery specifications and ordering information

When requesting a quote for the plasmid DNA preparation services, please specify:

  • Quantity of plasmid DNA: starting from 100 µg
  • Storage condition: water, TE buffer or dry
  • Quality grade: Research Grade or Transfection Grade

Quotations and Ordering

  • For quotation requests and questions about plasmid DNA preparation, you may contact us by phone, email, fax, or our secured online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.