Plasmid DNA Preparation Services |
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GenScript plasmid DNA preparation services cover individual constructs for research labs as well as large scale manufacturing for biotech and pharmaceutical companies. GenScript’s high quality plasmids will help you achieve highly efficient cell transfection, DNA vaccination, antibody production, and other preclinical studies.
GenScript USA Inc. has upgraded its plasmid DNA preparation platform. We are currently offering SC Grade and Advanced SC Grade (Supercoiled content ≥ 95%, with enzyme-free and animal-free process) plasmids along with routine Research Grade plasmid production to satisfy customers’ variable needs.
- Service Specifications
- Ordering Information
- Customer Testimonial
Quality Grades |
Features |
Typical Applications |
| Research Grade |
Predominantly supercoiled |
- Typical molecular biology research
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| SC Grade |
≥ 95% Supercoiled*
≤ 0.03 EU/μg Endotoxin |
- Recommended for effective transfection and co-transfection
- Antibody production
- Protein manufacturing
- Animal studies
- Gene vaccine and gene therapy studies
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| Advanced SC Grade |
≥ 95% Supercoiled*
≤ 0.005 EU/μg Endotoxin |
- Highly effective transfection and co-transfection
- Antibody production
- Protein manufacturing
- Animal studies
- Gene vaccine and gene therapy studies
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* Our standard supercoiled content is measured analytically by densitometry, of which the data obtained is for information only.
** Additional charges may be applied for unstable plasmid production (e.g. toxic plasmids).
Features:
- Flexible scale: up to gram level. Starting from $50.00 for a MidiPrep (100 μg)
- Completely enzyme-free and animal-free process for SC Grade and Advanced SC Grade plasmids' production.
- Stringent and comprehensive quality control: at least seven items, including appearance, A260/280, supercoiled content, residual RNA, genomic DNA, concentrations, restriction analysis, and endotoxin level (upon request).
- Fast delivery time.
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Ordering Information―Starting from $50.00 for a MidiPrep (100 μg)
GenScript's plasmid DNA preparation service provides plasmid DNAs with quantities ranging from milligram to gram level. Both high-copy and low-copy plasmid DNAs are available.
Sample Submission Requirements:
- Plasmid DNA template of > 1 μg with a concentration of > 100 ng/μl.
- Dissolved in water, TE buffer or dry in 1.5/0.5 ml centrifuge tube.
- Bacteria stock and plasmid DNA that is dry on paper are acceptable but not recommended.
Quality Control:
| QC Items |
Specification |
| Appearance |
Clear, no visible particles |
| A260/280 |
1.8–2.0 |
| Supercoiled content |
Based on the grade |
| Residual RNA |
Not visible on agarose gel |
| Genomic DNA |
Not visible on agarose gel |
| Concentrations |
0.5–4 mg/ml upon request |
| Restriction Analysis |
Conforms to reference |
| Endotoxin Assay (LAL test) |
Based on the grade |
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Customer Testimonial:
"We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!"
― Dr. Melissa A. Melan, University of Pittsburgh Medical Center, USA
Selected Publications:
- Charles K. Lee, S. Craig Cary, Alison E. Murray, and Roy M. Daniel. ENZYMOLOGY AND PROTEIN ENGINEERING:
Enzymic Approach to Eurythermalism of Alvinella pompejana and Its Episymbionts. Appl. Envir. Microbiol. Feb 2008; 74: 774–782
- Anxun Wang, Bin Zhang, Hongzhang Huang, Leitao Zhang, Donglin Zeng, Qian Tao, Jianguang Wang, and Chaobin Pan. Suppression of local invasion of ameloblastoma by inhibition of matrix metalloproteinase-2 in vitro. BMC Cancer. Jun 2008; 8: 182
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 Quotations and Ordering:
 For quotation requests and questions about plasmid DNA preparation, you may contact us by phone, email, fax, or via our secured online quotation system.
Orders of plasmid DNA preparation can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.
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