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plasmid preparation service

plasmid preparation serviceCustom Plasmid DNA Preparation Services

Flexible scale: 100 μg to 1,000 mg from high- or low-copy plasmids

High quality: Standard concentration of 1 mg/ml, predominantly supercoiled

Stringent quality control: A260/280 of 1.8-2.0; RNA and genomic DNA are undetectable, excellent homogeneity, endotoxin level below 5 EU/mg (upon request)

Competitive prices: Starting from $50 for a MidiPrep (100 μg)

"We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!"

― Dr. Melissa A. Melan, University of Pittsburgh Medical Center, USA

GenScript provides pure plasmid DNA from both high-copy and low-copy plasmids grown from bacterial cultures. These plasmids can be used for many applications within molecular biology, such as mammalian transfection, in vitro transcription and automated sequencing as well as standard downstream applications such as restriction analysis, labeling, ligation, cloning, probe construction, hybridization and PCR without further manipulation. Our protocols minimize DNA shearing and increase plasmid integrity. We also provide ultra-low endotoxin DNA for transfection and gene therapy studies.

Our plasmid preparation services cover individual constructs for research labs as well as large scale manufacturing for biotech suppliers and pharmaceutical companies. For biotech suppliers, we also provide complete vector production service (batch tube aliquoting, labeling, and QC).

  • Services
  • Track Record
Cat. No. ProductTurnaround Time
SC1323 Midi (100 μg) 1 week
SC1324 Maxi (200 μg) 1 week
SC1325 Maxi (500 μg) 1 week
SC1326 Mega (1 mg) 1 week
SC1089 Mega (2 mg) 1 week
SC1090 Giga (10 mg–High-copy) 2 weeks
SC1091 Giga (10 mg–Low-copy) 2 weeks
SC1092 20 mg (High-copy) 2 weeks
SC1093 20 mg (Low-copy) 2 weeks
SC1094 50 mg (High-copy) 2–3 weeks
SC1095 50 mg (Low-copy) 2–3 weeks
SC1096 100 mg (High-copy) 3–4 weeks
SC1097 100 mg (Low-copy) 3–4 weeks
SC1098 Customized Plasmid Preparation (up to 1,000 mg) Request a Quote

Additional Services:

  • Endotoxin Removal: Low endotoxin grade (< 30 EU/mg)
  • Endotoxin Removal: Ultralow endotoxin grade (< 5 EU/mg)
  • Bio-burden Assay
  • Endotoxin Assay (LAL test)
  • Restriction Analysis

Sample Submission Requirements:

  • Plasmid DNA template of > 1 μg with a concentration of > 100 ng/μl.
  • Dissolved in water, TE buffer or dry in 1.5/0.5ml centrifuge tube.
  • Bacteria stock and plasmid DNA that is dry on paper are acceptable but not recommended.

For more information, please call us at 1-732-885-9188, or email us at gene@genscript.com. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Recent publications

plasmid preparation service Charles K. Lee, S. Craig Cary, Alison E. Murray, and Roy M. Daniel. ENZYMOLOGY AND PROTEIN ENGINEERING: Enzymic Approach to Eurythermalism of Alvinella pompejana and Its Episymbionts. Appl. Envir. Microbiol. Feb 2008; 74: 774–782

plasmid preparation service Anxun Wang, Bin Zhang, Hongzhang Huang, Leitao Zhang, Donglin Zeng, Qian Tao, Jianguang Wang, and Chaobin Pan. Suppression of local invasion of ameloblastoma by inhibition of matrix metalloproteinase-2 in vitro. BMC Cancer. Jun 2008; 8: 182

What our customers say...

"I'm very satisfied with GenScript plamsid preparation, and the labelling is clear. I got ALL info I want & that was really happy experience. "

― J. Saucerman

"Everything went smoothly. Both plasmid prep and Southern Blots brought me good result for my experiment."

― M. A. Melan

"Perfect, no problem at all!"

― H. Wang

online quotationQuotations and Ordering

For quotation requests and questions, you may contact us by phone, email, fax, or via our online quotation system.

Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.

Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.

quote online Online Quotation
Order by email Email: gene@genscript.com
Order by phone Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Fax: 1-732-210-0262 1-732-885-5878