plasmid DNA preparation

Plasmid DNA Preparation Services

GenScript plasmid DNA preparation services are amenable to small research labs as well as large-scale manufacturing biotech and pharmaceutical companies. Our high-quality plasmids will help you achieve highly efficient cell transfection, as well as support DNA vaccine development, antibody production, and other preclinical research projects.

Our plasmid DNA preparation platform has recently been upgraded to provide high-quality plasmid preparation. Choose from our Research Grade and Transfection Grade packages (both employing animal-free and enzyme-free processes), to obtain the purified plasmid that best fits your research needs. Plasmid purification for both low-copy number and high-copy number plasmids are available.

Plasmid Prep Service Advantages

  • Economical: 100 µg miniprep starting from just $50
  • Save Time: Fast turnaround time
  • Quality Assured: Minimum of 7 stringent QC parameters analyzed for each prep
  • One-stop downstream solutions: Combine with gene synthesis, custom cloning, or mutagenesis

  • Service Specifications
  • Ordering Information
  • Customer Testimonial
Quality Grade
Research Grade
Transfection Grade
Features
  • Animal-free, enzyme-free process
  • Animal-free, enzyme-free process
  • Predominantly supercoiled
  • ≥ 95% supercoiled*
-
  • ≤ 0.005 EU/µg endotoxin levels
  • Bioburden assay analysis (optional)
  • Bioburden assay analysis
  • Sterile filtering (optional)
  • Sterile filtering (optional)
Applications
  • Molecular cloning
  • Sequencing
  • Mutagenesis
  • Southern blot
  • Library construction
  • Probe synthesis
  • Transfection or transformation (Recommended for E. coli, bacillus, mould, yeast)
  • Highly effective transfection and co-transfection (Recommended for mammalian cells)
  • Antibody production
  • Gene vaccine and gene therapy studies
  • Protein manufacturing
  • Animal studies
Deliverables
μg to g amounts of plasmid according to your specifications
Turnaround time
1 week for ≤ 1 mg (Research Grade)
QC Items
Appearance
A260/280
Supercoiled Content
Residual RNA
Genomic DNA
Concentrations
Restriction Analysis
Endotoxin Assay
(LAL test)
-
Bio-burden Assay
optional
* Our standard supercoiled content is measured analytically by densitometry, of which the data obtained is for information only.
** Additional charges may be applied for unstable plasmid production (e.g. toxic plasmids).

Ordering Information―Starting from $50.00 for a MidiPrep (100 μg)

GenScript's plasmid DNA preparation service provides plasmid DNAs with quantities ranging from milligram to gram level. Both high-copy and low-copy plasmid DNAs are available.

Sample Submission Requirements:

  • Plasmid DNA template of > 1 μg with a concentration of > 100 ng/μL.
  • Dissolved in water, TE buffer or dry in 1.5 mL or 0.5 mL centrifuge tube.
  • Bacteria stock and plasmid DNA that is dry on paper are acceptable but not recommended.

Quality Control

QC Items Research Grade Transfection Grade Specification
Appearance
Clear, no visible particles
A260/280
1.8–2.0
Supercoiled content
Based on the grade
Residual RNA
Not detectable on agarose gel
Genomic DNA
Not detectable on agarose gel
Concentrations
1 mg/mL by default, or any concentration between 0.5-4 mg/mL upon request
Restriction Analysis
Conforms to reference
Endotoxin Assay (LAL test)
-
Based on the grade
Bio-burden Assay
Optional
No growth on agar plate after 48 hours
*Sterile filtering is available at additional charge; request a quote.

Customer Testimonial

"We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!"

― Dr. Melissa A. Melan, University of Pittsburgh Medical Center, USA

Selected Publications

  • Ghezzi S, Galli L, Kajaste-Rudnitski A, et al. Identification of TRIM22 single nucleotide polymorphisms associated with loss of inhibition of HIV-1 transcription and advanced HIV-1 disease. AIDS. Sep 2013.

  • Waaijers S, Portegijs V, Kerver J, et al. CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013. doi:10.1534/genetics.113.156299.

  • Zhang X, Zhang J, Bauer A, Zhang L, Selinger DW, Lu CX, Ten Dijke P. Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6. EMBO J: 32, 996 - 1007 (1 March 2013) doi:10.1038/emboj.2013.38
  • See more recent peer-reviewed publications that cite GenScript's Plasmid Prep. Services


Quotations and Ordering

  • For quotation requests and questions about plasmid DNA preparation, you may contact us by phone, email, fax, or via our secured online quotation system.
  • Orders of plasmid DNA preparation can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.