Custom Polyclonal Antibody Services (Rat) |
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Services
*: Antigens supplied by clients. **: Peptide antigens designed, synthesized, and conjugated by GenScript
Standard Protocol (Three animals)
| Steps |
Time from Project Initiation |
Time from Immunization |
Activity Description |
| Peptide synthesis |
≈2 weeks |
- |
- |
| Peptide conjugation to carrier |
≈3 weeks |
- |
- |
| Control serum collection |
≈4 weeks |
Day 0 |
Pre-bleed (≈0.1 ml serum) |
| Primary immunization |
≈4 weeks |
Day 1 |
0.1 mg per rat peptide-KLH conjugate, CFA, i.p. |
| 1st boost |
≈6 weeks |
Day 14 |
0.1 mg per rat peptide-KLH conjugate, IFA, i.p. |
| Test bleed and ELISA |
≈8 weeks |
Day 24 |
0.01-0.02 ml serum per rat. ELISA and initial response evaluation. |
| 2nd boost |
≈9 weeks |
Day 35 |
0.1 mg per rat peptide-KLH conjugate, IFA, i.p. |
| Test bleed and ELISA |
≈11 weeks |
Day 45 |
0.01-0.02 ml serum per rat. ELISA and initial response evaluation. |
| 3rd boost |
≈12 weeks |
Day 56 |
0.1 mg per rat peptide-KLH conjugate, IFA, i.p. |
| Production bleed |
≈14 weeks |
Day 66 |
1.5 – 2 ml serum per rat |
| Lyophilization |
≈15 weeks |
Day73 |
- |
| ELISA and shipping |
≈16 weeks |
Day 80 |
Extend or terminate |
Note: All antisera or purified antibodies are provided as lyophilized form except special requirements. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.
Quotation and Ordering:
 To request a quotation, please download and complete our Quick Quotation Request. |
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| To order a pAb, please download & complete our Polyclonal Antibody Order Form. |
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| To submit an antigen, mail it together with a completed hard copy of Antigen Submission |
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| Form to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US. |
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Please email or fax all quotation requests or completed order forms (including the Antigen Submission Forms) to GenScript.
If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our Secure Web Server.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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