Custom Polyclonal Antibody Services (Rat) | | Peptide Supplied Polyclonal Antibody Package (Rat) (Cat. No.: SC1313) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Three rats, pre-immune bleed, four immunizations/rat
- ELISA test
- Guarantee Titer:>1:32,000
| - 50-100 μl of pre-immune serum from each rat
- ELISA results
- 5 ml of antisera from all rats
- 5 mg of unconjugated peptide, MS and HPLC report
| - Peptide synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Shipping: 1-2 weeks
- Total 14-16 weeks
| Complete Protein G-Purified Peptide Polyclonal Antibody Package (Rat) (Cat. No.: SC1314) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Three rats, pre-immune, four immunizations/rat
- Antibody purification by protein G column
- ELISA test
- Guarantee Titer:>1:32,000
| - 10-15 μl of pre-immune serum from each rat
- ELISA results
- 4.5-22.5 mg purified antibody from all antisera
- 5 mg of unconjugated peptide, MS and HPLC report
| - Peptide synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Shipping: 1-2 weeks
- Total 15-17 weeks
| Standard Protocol (Three animals) | Steps | Time from Project Initiation | Time from Immunization | Activity Description | | Peptide synthesis | ≈2 weeks | - | - | | Peptide conjugation to carrier | ≈3 weeks | - | - | | Control serum collection | ≈4 weeks | Day 0 | Pre-bleed (≈0.1 ml serum) | | Primary immunization | ≈4 weeks | Day 1 | 0.1 mg per rat peptide-KLH conjugate, CFA, i.p. | | 1st boost | ≈6 weeks | Day 14 | 0.1 mg per rat peptide-KLH conjugate, IFA, i.p. | | Test bleed and ELISA | ≈8 weeks | Day 24 | 0.01-0.02 ml serum per rat. ELISA and initial response evaluation. | | 2nd boost | ≈9 weeks | Day 35 | 0.1 mg per rat peptide-KLH conjugate, IFA, i.p. | | Test bleed and ELISA | ≈11 weeks | Day 45 | 0.01-0.02 ml serum per rat. ELISA and initial response evaluation. | | 3rd boost | ≈12 weeks | Day 56 | 0.1 mg per rat peptide-KLH conjugate, IFA, i.p. | | Production bleed | ≈14 weeks | Day 66 | 1.5 – 2 ml serum per rat | | ELISA and shipping | ≈15 weeks | Day73 | Extend or terminate | Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.Quotations and Ordering:   For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
 | Email: antibody@genscript.com |  | Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |  | Fax: 1-732-210-0262 1-732-885-5878 | |
