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Protein Expression and Purification Service Summary

Services Included:

1. Fermentation:

  • Large-scale culture of bacteria and yeast
2. Expression Construct Generation:
  • Gene synthesis and optimization
  • Subcloning of the gene for expression
  • Expression vector construction and preparation
3. Stable Cell Line Establishment:
  • High-expression cell lines for yeast, insect, and mammalian cells
4. Recombinant Baculovirus Generation
5. Baculovirus Stock and Insect Cell Pellet
6. 15N- and 13C- Labeled Protein Expression
7. Small-Scale Expression and Purification:
  • Optimization of expression conditions
  • Small-scale expression and purification
8. Large-Scale Production and Purification:
  • Large-scale production using fermentors, waveTM mixers, and bioreactors
  • Large-scale purification
9. Purification Options:
  • Affinity column
  • Hydrophobic column
  • Gel filtration
  • Ion exchange
10. Membrane Preparation
11. Large-Volume Transient Transfection
12. Large-Volume Frozen Transients for High-Throughput Screening (HTS)
13. Protein Characterization:
  • SDS-PAGE electrophoresis followed by Coomassie blue staining
  • Western blot analysis
  • Absorption spectrum
  • N-terminal sequencing
  • Protein concentration determinations
  • Enzyme activity assay
  • Mass spectrometry
  • NMR spectroscopy

14. Protein Refolding

Delivery Specifications:

Protein service deliverables include custom proteins, analysis data, QC reports, DNA constructs (optional), and cell lines (optional).

Quotations and Ordering:

To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure web server.

If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.

For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure web server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Order by mail Email: protein@genscript.com
Order by phone Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Fax: 1-732-210-0262 1-732-885-5878