 GenScript's siRNA Design Center
GenScript's proprietary OptimumRNAiTM technology offers 2nd
generation siRNA design tool, which can efficiently reduce off-target results and enhance the knockout rate.
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- siRNA Target Finder
- siRNA Construct Builder
- Sequence Scrambler
- Genome-Wide Design
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Use this tool to identify candidate siRNA target sequences within your
cDNA.
GenScript’s siRNA design tool is effective and easy to handle.
With the new tools, at least three out of four siRNA constructs will result in an mRNA knockdown rate of at least 75% where the transfection efficiency of the experiment is at least 80%.
The confidentiality of your sequence information is protected by Verisign Encryption Technology.
 siRNA Target Finder
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siRNA Construct Builder
Use this tool to build small hairpin inserts. GenScript precision gives your project the highest possible knockdown efficiency.
GenScript siRNA Construct Builder queries and processes information from a variety of databases, including not
only the public GenBank database but also GenScript's specialized proprietary expression vector, restriction endonuclease, and shRNA
loop databases. The GenScript siRNA Construct Builder provides analysis and calculation results tailored to the user's specific requirements,
providing four critical types of information: 1) Target shRNA: this is the sequence of sense siRNA, the sequence of the loop, and the sequence
of antisense siRNA. 2) Custom cloning sites (restriction sites) 3) Description of the expression vector selected by the user 4) The necessary
quantity of synthesized oligonucleotides.
siRNA Construct Builder |
siRNA Sequence Scrambler
This software creates a scrambled sequence as negative control for siRNA experiments.
The scrambled sequence will have the following features:
- It will have identical nucleotide composition to the input sequence.
- It will pass the same types of siRNA filtering (e.g. same level of complexity).
- It will have the minimum possible number of matches with any mRNA of that particular organism.
To identify a really good candidate, up to 10,000 scrambling combinations may be processed.
siRNA Sequence Scrambler
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Genome-Wide Design
GenScript’s Genome-Wide Bio-Reagent Bank (GenPool) includes 80,139 pre-designed siRNA designed for use with human, mouse, and rat genes.
Our design saves time and maximizes the chance of a successful experiment. GenScript's siRNA design algorithm includes analysis for sequence specificity, thermodynamic
properties, complexity, secondary structure, and SNPs.
Genome-Wide Design
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