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Endofree plasmid

Endofree Plasmid Maxiprep Kit

Endotoxin-free DNA plays an important role in gene therapy research and it is highly suited for a broad variety of demanding applications such as transfection, in vitro transcription and translation, and all enzymatic modifications. Ion-exchange based QuickClean EndoFree Plasmid Maxiprep Kit provides a single-step & rapid endotoxin-free DNA purification from E. coli host strains within 75 mins. The purified DNA is compatible for the downstream applications in transfection, in vitro transcription, translation, and enzymatic modifications. The endotoxin concentration in purified plasmid DNA is guaranteed less than 0.02 EU/μg.

QuickClean Endofree Plasmid Maxiprep Kit Can Provide:

Fast and easy process within 75 minutes
High Binding Capacity: more than 500 μg of plasmid DNA.
Final Endotoxin levels of plasmid DNA: < 0.02 EU/μg
Enhanced transfection efficiency than other companies

QuickClean Endo-free Plasmid Maxiprep Kit can purify plasmid DNA for downstream applications.

Transfection
In vitro transcription
Restriction analysis, ligation, labeling, etc.
Sequencing
Gene therapy

	Cat. No.	Product Name	Size	Price	Quantity
	L00421-10	QuickClean EndoFree Plasmid Maxiprep Kit	10 preps	$198
	L00421-5	QuickClean EndoFree Plasmid Maxiprep Kit	5 preps	$109

Specialty
Examples
Related Products

Procedure At-A-Glance GenScript Endo-free plasmid maxiprep kit provides fast anion-exchange-based endotoxin-free plasmid DNA purification. All key procedure will generally take approximately less than 1.5 hrs (see right), and the final endotoxin level can be less than 0.02 EU/μg.

The characteristics of EndoFree Plasmid Maxiprep Column:
Culture Volume	100 - 300 ml
Ligand	Anion-exchange chromatography
Binding capacity	>500 μg transfection grade plasmid or cosmid DNA
Matrix spherical	Silica gel
Particle size	74-154 μm
Final endotoxin level	< 0.02 EU/μg of DNA
Note: To prevent cross-contamination, it is not intended for repeat use.

Components	QuickClean EndoFree Plasmid Maxiprep Kit
Reactions	5 rxns	10 rxns
EndoFree Plasmid Maxiprep Column	5	10
Prep Buffer 1	55 ml	110 ml
Prep Buffer 2	55 ml	110 ml
Prep Buffer 3	55 ml	110 ml
Equilibration Buffer	55 ml	110 ml
Endotoxin Eraser Buffer*	210 ml	2 x 210 ml
Wash Buffer*	210 ml	2 x 210 ml
Elution Buffer*	80 ml	160 ml
Endotoxin-free Water	8 ml	15 ml
Endotoxin-free TE buffer*	8 ml	15 ml
RNase A solution(10 mg/ml)	0.55 ml	1.1 ml
Endotoxin-free pipette tips (1 ml)	18 tips	30 tips
Endotoxin-free Collection Tube (50 ml)	5 tubes	10 tubes
Endotoxin-free Collection Tube (1.5 ml)	10 tubes	20 tubes
* The endotoxin level of buffers is less than 0.01 EU/ml. To avoid cross-contamination, use this buffers provided with the QuickClean EndoFree Plasmid Maxiprep Kit.

High DNA Yield & Removal Efficiency

Figure 1. Enhanced plasmid DNA yields and Lower endotoxin level after purification: GenScript VS Competitor A

Good Performance - High Purity

Figure 2. Agarose gel analysis of a plasmid purification procedure using QuickClean EndoFree Plasmid Maxiprep Kit. 2 μl of sample was run on a 1% agarose gel. Lane 1 and 6: DNA KB Ladder Lane 2. Lysate added Prep Buffer 3 which contains supercoiled and open circular plasmid DNA Lane 3. Flow through fraction after plasmid DNA is bound to Column Lane 4. Flow through after wash the DNA bound column Lane 5. Purified plasmid DNA

High Transfection Efficiency: GenScript VS Company A

Figure 3. Transfection of the recombinant plasmid containing human IgG1 antibody was conducted using DNA purified with EndoFree Plasmid Maxiprep Kit (GenScript) and Company A's maxiprep kit, respectively. The cell culture supernatant samples from 3 to 6 days post-transfection were analyzed by ELISA. The primary antibody was Goat Anti-Human IgG Antibody (H&L) [HRP], pAb (Cat.No. A00166, GenScript)..

	Cat. No.	Product Name	Size	Price	Quantity
	L00420-100	QuickClean II Plasmid Miniprep Kit	100 rxns	$85
	L00420-50	QuickClean II Plasmid Miniprep Kit	50 rxns	$45
	L00418-100	QuickClean II Gel Extraction Kit	100 rxns	$85
	L00418-50	QuickClean II Gel Extraction Kit	50 rxns	$45
	L00419-100	QuickClean II PCR Extraction Kit	100 rxns	$85
	L00419-50	QuickClean II PCR Extraction Kit	50 rxns	$45
	L00237	QuickClean 96-well Plasmid Miniprep Kit	384 Prep	$488
	L00238	QuickClean 96-Well PCR Purification Kit	384 Prep	$295