ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit: The kit utilizes a modified Limulus Amebocyte Lysate and a synthetic color producing substrate to quantitatively detect endotoxin chromogenically in a broad range of 0.005 - 1 EU/ml.
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Technical Advantages:
Under the standard conditions, the absorbance at 545 nm is linear in the concentration range of 0.005 to 0.1 or 0.1 to 1 EU/ml endotoxin. Plot the mean Δabsorbance for the four standards on the y-axis vs the corresponding endotoxin concentration in EU/ml on the x-axis. Draw a best fit straight line between these points and determine endotoxin concentrations of samples graphically. See the following figure.
ToxinSensorTM Gel Clot Endotoxin Assay Kit: It is a qualitative test for Gram-negative bacterial endotoxin. Limulus Amebocyte Lysate as supplied is to be reconstituted with LAL Reagent Water and then mixed in equal parts with the solution being tested. After incubation, and in the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur.
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Technical Advantages:
- Sample: Protein A, 1 mg/ml in PBS, pH 7.4. It is purified from E. coli sonicate by Ni-NTA resin.
- Dilution by LAL water: 1: 200,000, 1: 400,000, 1: 800,000
- The test is performed as described above and the assay result is:
Positive control |
Negative control |
1: 200,000 |
1: 400,000 |
1: 800,000 |
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