Catalog Products » Other Products » Endotoxin Detection and Removal System

Endotoxin Detection & Removal

Endotoxin Detection & Removal System

Endotoxin is a major contaminant found in biologically active substances. The presence of endotoxin can cause pyrogenic reactions in host organisms such as endotoxin shock, tissue injury, and even death. Endotoxin detection test is one of the most critical quality control tests required by the FDA for all drugs in their final formulation. Therefore, it is essential to remove endotoxin from human and animal parenteral drugs, biological products, and medical devices.

ToxinSensor™ Endotoxin Detection System uses FDA approved LAL (Limulus Amebocyte Lysate) testing methods to achieve a fast and highly sensitive endotoxin assay. The Chromogenic LAL Endotoxin Assay kit can quantitatively detect endotoxin in a broad range (0.005–1 EU/ml). The Gel Clot Endotoxin Assay kit is a fast qualitative test showing a positive or negative result. ToxinEraser™ Endotoxin Removal System facilitates the endotoxin removal process with a high binding capacity (> 2,000,000 EU/ml).

  • Endotoxin Detection
  • Endotoxin Removal
  • FAQ
  • Customer Citations

ToxinSensor™ Endotoxin Detection System

   —To ensure accurate results with rapid endotoxin assay method!

  • Delivered with ready-to-use reagents and materials
  • Strong linearity and reproducibility
  • Guaranteed high-sensitivity: 0.005 EU/ml

ToxinSensor™ Endotoxin Assay Kits can be widely used in in vitro end-product endotoxin tests, including those for human and animal parenteral drugs, biological products, and medical devices.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00350C ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit
1 Kit (16 rxns)
$80.00
L00350 ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit
1 Kit (32 rxns)
$150.00
L00351 ToxinSensorTM Gel Clot Endotoxin Assay Kit
1 Kit
$90.00
L00447-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.015 EU/ml
$150.00
L00447-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.015 EU/ml
$80.00
L00448-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.03 EU/ml
$150.00
L00448-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.03 EU/ml
$80.00
L00449-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.06 EU/ml
$150.00
L00449-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.06 EU/ml
$80.00
L00450-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.125 EU/ml
$150.00
L00450-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.125 EU/ml
$80.00
L00451-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.25 EU/ml
$150.00
L00451-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.25 EU/ml
$80.00

Accessory Products

Cat. No.
Product Name
Size
Price
Quantity
L00402 ToxinEraserTM Endotoxin Removal Resin
1 ml
$60.00
M01072-5 ToxinSensorTM Endotoxin-free Tubes
5 Tubes
$20.00
M01072-40 ToxinSensorTM Endotoxin-free Tubes
40 Tubes
$100.00
M01072-10 ToxinSensorTM Endotoxin-free Tubes
10 Tubes
$33.00
M01063 ToxinSensorTM Endotoxin-free Pipette Tips (1 ml, Blue)
1 PK of 6 tips
$6.00
M01053 ToxinEraserTM Regeneration Buffer
125 ml
$25.00
M01054 ToxinEraserTM Equilibration Buffer
125 ml
$25.00

ToxinEraser™ Endotoxin Removal System

   —For efficient removal of endotoxin from samples! For research only.
  • High binding capacity at least 2, 000, 000 EU / ml (CV)
  • High Recovery Yield: >90% with minimized sample loss
  • High stability and high removal efficiency: Bring down endotoxin level to as low as 0.1 EU/ml*.

* With repeat use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

GenScript ToxinEraser™ is an efficient endototoxin removal tool. The final endotoxin level can be reduced below 1 EU/mg with repeated use of ToxinEraser™ Advanced Endotoxin Removal resin. These kits can be used to remove endotoxin from proteins, peptides, antibodies, or even DNA samples.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00338 ToxinEraserTM Endotoxin Removal Kit
1 kit
$110.00

Accessory Products

Cat. No.
Product Name
Size
Price
Quantity
L00402 ToxinEraserTM Endotoxin Removal Resin
1 ml
$60.00
M01072-5 ToxinSensorTM Endotoxin-free Tubes
5 Tubes
$20.00
M01072-40 ToxinSensorTM Endotoxin-free Tubes
40 Tubes
$100.00
M01072-10 ToxinSensorTM Endotoxin-free Tubes
10 Tubes
$33.00
M01063 ToxinSensorTM Endotoxin-free Pipette Tips (1 ml, Blue)
1 PK of 6 tips
$6.00
M01053 ToxinEraserTM Regeneration Buffer
125 ml
$25.00
M01054 ToxinEraserTM Equilibration Buffer
125 ml
$25.00

Selection Guide

  ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit ToxinSensor™ Gel Clot Endotoxin Assay Kit ToxinSensor™ Single Test Kit
General Introduction This kit utilizes chromogenic LAL assay to quantitatively detect endotoxin in a broad range. This kit is a convenient endotoxin qualitative test based on gelation principle. This kit is a one-step qualitative endotoxin test; various sensitivities are available.
Application Accurately determine endotoxin levels End-product endotoxin qualitative detection One-step end-product endotoxin qualitative detection
Sensitivity 0.005–1 EU/ml 0.25 EU/ml 0.015 EU/ml- 0.25 EU/ml
Reconstitute LAL with LAL Reagent Water
Yes
Yes
No
Need Spectrophotometer
Yes
No
No

Kit Components
  • Ready-to-use reagents
  • Endotoxin-free tips & tubes
  • Ready-to-use reagents
  • Endotoxin-free tips & tubes
  • Ready-to-use reagents
Sample Requirement Colorless and Clear liquid N/A N/A
Size L00350C 16 rxns
L00350 32 rxns
L00351 40 assays L00447 - L00451
20/ 40 assays
  1. How to run an endotoxin detection assay for medical devices?

    Infuse 15 ml of endotoxin-free water into the lumen of medical device (e.g. infusion apparatus, dialysis tubing, etc.) and shocks five times, seal all the ends and incubate it at 37°C water bath for 2 hours, then transfer the water to an endotoxin-free vial. Detect the endotoxin concentration in the water by using ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350), and the total endotoxin value in the water can be determined.

  2. Can I use plastic ware when I prepare standard endotoxin solution?

    Endotoxins adhere strongly to glassware and are difficult to remove completely during washing. Standard laboratory autoclaving procedures have little or no effect on endotoxin levels. Heating glassware at 180°C overnight is recommended to destroy any attached endotoxin molecules. We recommend you to only use new plastic ware tubes which are certified to be endotoxin-free when you prepare standard endotoxin solution. GenScript supplies ToxinSensor™ Endotoxin-free Tubes (Cat. No. M01072) for you to dilute or aliquot samples. Endotoxin adheres to plastic surfaces more strongly than glass surfaces.

  3. Is standard endotoxin solution reusable after developed (color formed)?

    No. Standard endotoxin solution should be prepared fresh for each test.

  4. How to determine the reliability of the final data?

    Firstly, we recommend you to prepare all materials included in the kit in a laminar flow cabinet and avoid contamination during assay process.
    For ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350), if the OD value of your sample is in the range of the standards, the final data will be reliable after you obtain a good linearity (R≥0.980).
    For ToxinSensor™ Gel Clot Endotoxin Assay Kit (Cat. No.L00351), you can obtain the reliable final result through negative and positive controls. If you get a positive result in the negative control, it may indicate that the LAL or LAL reagent water has been contaminated, or if you get a negative result in the positive control, it may indicate that the LAL reagent has lost activity.

  5. Can I use 96-well microplate to read the absorbance of each reaction at 545 nm?

    ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350) is designed to deliver high sensitivity quantitative assay in tubes, and not intended to be used for a 96-well plate assay. However, you can perform the assay in tubes, after color development, transfer 200 µl of the final solution into an endotoxin-free 96-well plate to read the result.

  6. Read more

  • Bergenfelz C., et al. Wnt5a inhibits human monocyte derived myeloid dendritic cell generation. Scand J Immunol. 2013 Aug;78(2):194-204.
  • Shalaby KH., et al. Inhaled Birch Pollen Extract Induces Airway Hyperresponsiveness via Oxidative Stress but Independently of Pollen-Intrinsic NADPH Oxidase Activity, or the TLR4-TRIF Pathway. J Immunol. 2013 Jul 15;191(2):922-33.
  • Clark KD., et al. Hemolymph melanization in the silkmoth Bombyx mori involves formation of a high molecular mass complex that metabolizes tyrosine. J Biol Chem. 2013 May 17;288(20):14476-87.
  • Müller M., et al. Alterations in the secretory pattern of dermal dendritic cells following melanin uptake. Cell Tissue Res. 2013 Jun;352(3):599-610.
  • Kim EH., et al. Prokaryote-expressed M2e protein improves H9N2 influenza vaccine efficacy and protection against lethal influenza a virus in mice. Virol J. 2013 Apr 3;10:104.
  • O'Meara CP., et al. Immunization with a MOMP-Based Vaccine Protects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology. PLoS One. 2013 Apr 16;8(4):e61962.
  • Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
  • Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
  • Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
  • Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
  • A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
  • Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
  • Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
  • K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
  • Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
  • Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
  • Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
  • Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
  • Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
  • Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
  • Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
  • Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
  • Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.
  • Kovacs-Nolan J., et al. β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. J Nutr. 2013 Mar;143(3):384-91.
  • Schepetkin IA., et al. Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. Int Immunopharmacol. 2013 Mar;15(3):628-37.
  • Ambalavanan N., et al. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L152-61.
  • Mishra B., et al. A novel antimicrobial peptide derived from modified N-terminal domain of bovine lactoferrin: design, synthesis, activity against multidrug -resistant bacteria and Candida. Biochim Biophys Acta. 2013 Feb;1828(2):677-86.
  • A Mountney, et al. Sialidase, Chondroitinase Abc And Combination Therapy After Spinal Cord Contusion Injury. J Neurotrauma. 2013 Feb 1;30(3):181-90.
  • Bastiaan-Net S., et al. Biochemical And Functional Characterization Of Recombinant Fungal Immunomodulatory Proteins (Rfips). Int Immunopharmacol. 2013 Jan;15(1):167-75.
  • Sameera Sayeed, et al. Multifunctional Role Of Human Splunc1 In Pseudomonas Aeruginosa Infection. Infect Immun. 2013 Jan;81(1):285-91.
  • K Kouakou, et al. Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia. J Ethnopharmacol. 2013 Mar 7;146(1):232-42.
  • Kaliannan K., et al. Intestinal alkaline phosphatase prevents metabolic syndrome in mice. Proc Natl Acad Sci USA. 2013 Apr 23;110(17):7003-8.
  • Gomez G., et al. Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach. PLOS ONE. 2013;8(3):e59751.
  • Chen H., et al. In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice. PLOS ONE. 2013;8(2):e58208.
  • Moshiri A., et al. Role of Tissue-Engineered Artificial Tendon in Healing of a Large Achilles Tendon Defect Model in Rabbits. J Am Coll Surg. 2013 Jun 29. pii: S1072-7515(13)00312-8.
  • Moshiri A., et al. Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits. J Tissue Eng Regen Med. 2013 May 2. doi: 10.1002/term.1740.
  • Chellan B., et al. LIGHT/TNFSR14 Can Regulate Hepatic Lipase Expression by Hepatocytes Independent of T Cells and Kupffer Cells. PLoS One. 2013;8(1):e54719.
  • Hao J., et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner.Cell Mol Immunol. 2013 Oct 7. doi: 10.1038/cmi.2013.44.
  • Sadraeian M., et al. Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from Shigella dysenteriae. Cell J. 2013 Jul;15(2):176-81.
  • Tripathi A., et al. Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification. Biochemistry. 2013 Jun 18;52(24):4184-92.