Catalog Products » Other Products » Endotoxin Detection and Removal System

Endotoxin Detection & Removal

Endotoxin Detection & Removal System

Endotoxin is a major contaminant found in biologically active substances. The presence of endotoxin can cause pyrogenic reactions in host organisms such as endotoxin shock, tissue injury, and even death. Endotoxin detection test is one of the most critical quality control tests required by the FDA for all drugs in their final formulation. Therefore, it is essential to remove endotoxin from human and animal parenteral drugs, biological products, and medical devices.

ToxinSensor™ Endotoxin Detection System uses FDA approved LAL (Limulus Amebocyte Lysate) testing methods to achieve a fast and highly sensitive endotoxin assay. The Chromogenic LAL Endotoxin Assay kit can quantitatively detect endotoxin in a broad range (0.005–1 EU/ml). The Gel Clot Endotoxin Assay kit is a fast qualitative test showing a positive or negative result. ToxinEraser™ Endotoxin Removal System facilitates the endotoxin removal process with a high binding capacity (> 2,000,000 EU/ml).

  • Endotoxin Detection
  • Endotoxin Removal
  • FAQ
  • Customer Citations

ToxinSensor™ Endotoxin Detection System

   —To ensure accurate results with rapid endotoxin assay method!

  • Delivered with ready-to-use reagents and materials
  • Strong linearity and reproducibility
  • Guaranteed high-sensitivity: 0.005 EU/ml

ToxinSensor™ Endotoxin Assay Kits can be widely used in in vitro end-product endotoxin tests, including those for human and animal parenteral drugs, biological products, and medical devices.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00350C ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit
1 Kit (16 rxns)
$90.00
L00350 ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit
1 Kit (32 rxns)
$170.00
L00351 ToxinSensorTM Gel Clot Endotoxin Assay Kit
1 Kit
$103.00
L00447-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.015 EU/ml
$170.00
L00447-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.015 EU/ml
$90.00
L00448-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.03 EU/ml
$170.00
L00448-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.03 EU/ml
$90.00
L00449-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.06 EU/ml
$170.00
L00449-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.06 EU/ml
$90.00
L00450-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.125 EU/ml
$170.00
L00450-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.125 EU/ml
$90.00
L00451-40 ToxinSensorTM Single Test Kit
1 kit (40 assay) 0.25 EU/ml
$170.00
L00451-20 ToxinSensorTM Single Test Kit
1 kit (20 assay) 0.25 EU/ml
$90.00

Accessory Products

Cat. No.
Product Name
Size
Price
Quantity
L00402 ToxinEraserTM Endotoxin Removal Resin
1 ml
$60.00
M01072-5 ToxinSensorTM Endotoxin-free Tubes
5 Tubes
$20.00
M01072-40 ToxinSensorTM Endotoxin-free Tubes
40 Tubes
$100.00
M01072-10 ToxinSensorTM Endotoxin-free Tubes
10 Tubes
$33.00
M01063 ToxinSensorTM Endotoxin-free Pipette Tips (1 ml, Blue)
1 PK of 6 tips
$6.00
M01053 ToxinEraserTM Regeneration Buffer
125 ml
$25.00
M01054 ToxinEraserTM Equilibration Buffer
125 ml
$25.00

ToxinEraser™ Endotoxin Removal System

   —For efficient removal of endotoxin from samples! For research only.
  • High binding capacity at least 2, 000, 000 EU / ml (CV)
  • High Recovery Yield: >90% with minimized sample loss
  • High stability and high removal efficiency: Bring down endotoxin level to as low as 0.1 EU/ml*.

* With repeat use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

GenScript ToxinEraser™ is an efficient endototoxin removal tool. The final endotoxin level can be reduced below 1 EU/mg with repeated use of ToxinEraser™ Advanced Endotoxin Removal resin. These kits can be used to remove endotoxin from proteins, peptides, antibodies, or even DNA samples.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00338 ToxinEraserTM Endotoxin Removal Kit
1 kit
$126.00

Accessory Products

Cat. No.
Product Name
Size
Price
Quantity
L00402 ToxinEraserTM Endotoxin Removal Resin
1 ml
$60.00
M01072-5 ToxinSensorTM Endotoxin-free Tubes
5 Tubes
$20.00
M01072-40 ToxinSensorTM Endotoxin-free Tubes
40 Tubes
$100.00
M01072-10 ToxinSensorTM Endotoxin-free Tubes
10 Tubes
$33.00
M01063 ToxinSensorTM Endotoxin-free Pipette Tips (1 ml, Blue)
1 PK of 6 tips
$6.00
M01053 ToxinEraserTM Regeneration Buffer
125 ml
$25.00
M01054 ToxinEraserTM Equilibration Buffer
125 ml
$25.00

Selection Guide

  ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit ToxinSensor™ Gel Clot Endotoxin Assay Kit ToxinSensor™ Single Test Kit
General Introduction This kit utilizes chromogenic LAL assay to quantitatively detect endotoxin in a broad range. This kit is a convenient endotoxin qualitative test based on gelation principle. This kit is a one-step qualitative endotoxin test; various sensitivities are available.
Application Accurately determine endotoxin levels End-product endotoxin qualitative detection One-step end-product endotoxin qualitative detection
Sensitivity 0.005–1 EU/ml 0.25 EU/ml 0.015 EU/ml- 0.25 EU/ml
Reconstitute LAL with LAL Reagent Water
Yes
Yes
No
Need Spectrophotometer
Yes
No
No

Kit Components
  • Ready-to-use reagents
  • Endotoxin-free tips & tubes
  • Ready-to-use reagents
  • Endotoxin-free tips & tubes
  • Ready-to-use reagents
Sample Requirement Colorless and Clear liquid N/A N/A
Size L00350C 16 rxns
L00350 32 rxns
L00351 40 assays L00447 - L00451
20/ 40 assays
  1. How to run an endotoxin detection assay for medical devices?

    Infuse 15 ml of endotoxin-free water into the lumen of medical device (e.g. infusion apparatus, dialysis tubing, etc.) and shocks five times, seal all the ends and incubate it at 37°C water bath for 2 hours, then transfer the water to an endotoxin-free vial. Detect the endotoxin concentration in the water by using ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350), and the total endotoxin value in the water can be determined.

  2. Can I use plastic ware when I prepare standard endotoxin solution?

    Endotoxins adhere strongly to glassware and are difficult to remove completely during washing. Standard laboratory autoclaving procedures have little or no effect on endotoxin levels. Heating glassware at 180°C overnight is recommended to destroy any attached endotoxin molecules. We recommend you to only use new plastic ware tubes which are certified to be endotoxin-free when you prepare standard endotoxin solution. GenScript supplies ToxinSensor™ Endotoxin-free Tubes (Cat. No. M01072) for you to dilute or aliquot samples. Endotoxin adheres to plastic surfaces more strongly than glass surfaces.

  3. Is standard endotoxin solution reusable after developed (color formed)?

    No. Standard endotoxin solution should be prepared fresh for each test.

  4. How to determine the reliability of the final data?

    Firstly, we recommend you to prepare all materials included in the kit in a laminar flow cabinet and avoid contamination during assay process.
    For ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350), if the OD value of your sample is in the range of the standards, the final data will be reliable after you obtain a good linearity (R≥0.980).
    For ToxinSensor™ Gel Clot Endotoxin Assay Kit (Cat. No.L00351), you can obtain the reliable final result through negative and positive controls. If you get a positive result in the negative control, it may indicate that the LAL or LAL reagent water has been contaminated, or if you get a negative result in the positive control, it may indicate that the LAL reagent has lost activity.

  5. Can I use 96-well microplate to read the absorbance of each reaction at 545 nm?

    ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Cat. No. L00350) is designed to deliver high sensitivity quantitative assay in tubes, and not intended to be used for a 96-well plate assay. However, you can perform the assay in tubes, after color development, transfer 200 µl of the final solution into an endotoxin-free 96-well plate to read the result.

  6. Read more

  • Jeong TH., et al. Soluble Expression and Partial Purification of Recombinant Human Erythropoietin from E. coli. Protein Expr Purif. 2014 Mar;95:211-8.
  • Belcher JD., et al. Heme triggers TLR4 signaling leading to endothelial cell activation and vaso-occlusion in murine sickle cell disease. Blood. 2014 Jan 16;123(3):377-90.
  • Carey AJ., et al. Infection and Cellular Defense Dynamics in a Novel 17β-Estradiol Murine Model of Chronic Human Group B Streptococcus Genital Tract Colonization Reveal a Role for Hemolysin in Persistence and Neutrophil Accumulation. J Immunol. 2014 Feb 15;192(4):1718-31.
  • Tabandeh MR., et al. Polysaccharides of Aloe vera induce MMP-3 and TIMP-2 gene expression during the skin wound repair of rat. Int J Biol Macromol. 2014 Apr;65:424-30.
  • Kuwabara T., et al. Predictive Significance of Kidney Myeloid-Related Protein 8 Expression in Patients with Obesity-or Type 2 Diabetes-Associated Kidney Diseases. PLoS One. 2014 Feb 18;9(2):e88942.
  • Zosky GR., et al. The Concentration of Iron in Real-World Geogenic PM10 Is Associated with Increased Inflammation and Deficits in Lung Function in Mice. PLoS One. 2014 Feb 28;9(2):e90609.
  • McGill JL., et al. Specific Recognition of Mycobacterial Protein and Peptide Antigens by {gamma}{delta} T Cell Subsets following Infection with Virulent Mycobacterium bovis. J Immunol. 2014 Mar 15;192(6):2756-69.
  • Caitlin A., et al. A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide. elife. 2014 Mar 4;3:e01579.
  • Imamura Y., et al. Salivary histatin 3 inhibits heat shock cognate protein 70-mediated inflammatory cytokine production through toll-like receptors in human gingival fibroblasts. J Inflamm (Lond). 2014 Feb 4;11(1):4.
  • Bourgeois B1., et al. The influence of Hurricanes Katrina and Rita on the inflammatory cytokine response and protein expression in A549 cells exposed to PM2.5 collected in the Baton Rouge-Port Allen industrial corridor of Southeastern. Toxicol Mech Methods. 2014 Mar;24(3):220-42.
  • Hendrickx DA., et al. Enhanced uptake of multiple sclerosis-derived myelin by THP-1 macrophages and primary human microglia. J Neuroinflammation. 2014 Mar 31;11:64.
  • Osman A., et al. M-CSF Inhibits Anti–HIV-1 Activity of IL-32, but They Enhance M2-like Phenotypes of Macrophages. J Immunol. 2014 Jun 1;192(11):5083-9.
  • Sakai Y., et al. Calcium Phosphate Particles Induce Interleukin-8 Expression in Human Gingival Epithelial Cell Line via Nuclear Factor-κB Signaling Pathway. J Periodontol. 2014 Apr 23.
  • Cardoso FM., et al. Single domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge. J Virol. 2014 Aug 1;88(15):8278-96.
  • Bedi D., et al. Selection of pancreatic cancer cell-binding landscape phages and their use in development of anticancer nanomedicines. Protein Eng Des Sel. 2014 Jul;27(7):235-43.
  • Kocher J., et al. Intranasal P particle vaccine provided partial cross-variant protection against human GII. 4 norovirus diarrhea in gnotobiotic pigs. J Virol. 2014 Sep 1;88(17):9728-43.
  • Reber LL., et al. Mast cell‐derived IL‐1β contributes to uric acid crystal‐induced acute arthritis in mice. Arthritis Rheumatol. 2014 Jun 18.
  • Sousa A., et al. Histamine monolith versatility to purify supercoiled plasmid deoxyribonucleic acid from Escherichia coli lysate. J Chromatogr A. 2014 Aug 15;1355:125-33.
  • Shi M., et al. Myeloid-derived suppressor cell function is diminished in aspirin-triggered allergic airway hyperresponsiveness in mice. J Allergy Clin Immunol. 2014 Jun 17.
  • Mac Sharry J., et al. Concomitant Exposure to Ovalbumin and Endotoxin Augments Airway Inflammation but Not Airway Hyperresponsiveness in a Murine Model of Asthma. PLoS One. 2014 Jun 26;9(6):e98648.
  • Jung ID., et al. Enhancement of Tumor-Specific T Cell–Mediated Immunity in Dendritic Cell–Based Vaccines by Mycobacterium tuberculosis Heat Shock Protein X. J Immunol. 2014 Aug 1;193(3):1233-45.
  • Wang Q., et al. MPT64 Protein from Mycobacterium tuberculosis Inhibits Apoptosis of Macrophages through NF-kB-miRNA21-Bcl-2 Pathway. PLoS One. 2014 Jul 7;9(7):e100949.
  • Phongsisay V., et al. LMIR5 extracellular domain activates myeloid cells through Toll-like receptor 4. Mol Immunol. 2014 Jul 5;62(1):169-177.
  • Letsiou E., et al. Pathologic Mechanical Stress and Endotoxin Exposure Increases Lung Endothelial Microparticle Shedding. Am J Respir Cell Mol Biol. 2014 Jul 16.
  • Guo X., et al. Adiponectin retards the progression of diabetic nephropathy in db/db mice by counteracting angiotensin II. Physiological Reports. 2014 Feb 10;2(2):e00230.
  • GARCIA-CONTRERAS R., et al. Induction of Prostaglandin E2 Production by TiO2 Nanoparticles in Human Gingival Fibroblast. In Vivo. 2014 Mar-Apr;28(2):217-22.
  • Kutsukake M., et al. Pioglitazone attenuates lung injury by modulating adipose inflammation. J Surg Res. 2014 Jun 15;189(2):295-303.
  • Laetitia R., et al. Microencapsulated Bifidobacterium longum subsp. infantis ATCC 15697 favorably modulates gut microbiota and reduces circulating endotoxins in F344 rats. Biomed Res Int. 2014 ;2014:602832.