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Cat. No.
 Name
Size   
Price
 Selected
E00007-1000
 Taq DNA Polymerase
1000 U
$60
E00007-50000
 Taq DNA Polymerase
50000 U
$2700
E00043
 Green Taq DNA Polymerase
1000 U
$60

Taq DNA Polymerase


Key Feature
  • Terminal Transferase Activity: Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
  • High-purity: No contamination activity has been detected in standard test reactions.

Description
Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR*.

  • Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).

    Applications
    The applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing


    Formulation
    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Concentration:
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    QC Tests
    PCR* performance, activity, nuclease
    Lane Taq Unit
    1Leading
    Brand A    		0.1
    2 0.25
    3 0.5
    4Leading
    Brand B    		0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5

    Store at -20.000 C


    Document


    References

    • Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005;
    • Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365


    Related Products
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    Price
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    D0056
    		 dNTP mixture, 10 mM each
    0.500 ml
    $25
    E00008
    		 Taq DNA Polymerase without Mg2+
    1000 U
    $60
    E00043
    		 Green Taq DNA Polymerase
    1000 U
    $60
    E00101
    		 1000 U of Taq (E00007); 0.5 ml of 10 mM dNTP (D0056)
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    $78
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