molecular biology services
Cat. No.
Name
Size   
Price
Selected
E00043
1000 U
$60
E00007-50000
50000 U (50 x 1000 U)
$2,700
E00007-1000
1000 U
$60

Taq DNA Polymerase IS a thermostable DNA Polymerase isolated FROM an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase IS the most common polymerase used FOR PCR.

  • Note: This product IS supplied WITH 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see ApplicationsThe applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing


    Formulation
    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Concentration:
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    QC Tests
    PCR* performance, activity, nuclease


    Lane Taq Unit
    1Leading
    Brand A
    0.1
    2 0.25
    3 0.5
    4Leading
    Brand B
    0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5

    Storage
    Store the product at -20°C.

    * The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.


    Document


    References

    • Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005;
    • Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365
    • Wang X., et al. Salicylic Acid Regulates Plasmodesmata Closure during Innate Immune Responses in Arabidopsis. Plant Cell. 2013 Jun;25(6):2315-29.
    • Ruth B. Phillips., et al. Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome. Int J Genomics. 2013 Mar;261730.
    • FrÍas M., et al. The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif in the protein surface. Mol Plant Pathol. 2013 Qct;12097.
    • Y Acanda., et al. EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine. Plant Cell Rep. 2013 Dec.
    • Firlej A., et al. A Multi-Approach Study to Delineate Interactions Between Carabid Beetles and Soybean Aphids. Environ Entomol. 2013 Feb;42(1):89 - 96.
    • Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol. 2013 Mar;27(3):511 - 523.
    • Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One. 2013 Jan;8(1):e50627.
    • Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A. 2013 Mar;110(10):3943 - 3948.
    • Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One. 2013 May;6(5):e62394.
    • Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb). 2013 Jul;111(1):16-22.
    • Carol D. von Dohlen., et al. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid (Adelges tsugae)(Hemiptera: Adelgidae) from its native and introduced range. Environ Microbiol. 2013 Jul;15(7):2043-62.
    • Gandhirajan RK., et al. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation. J Clin Invest. 2013 Feb;123(2):887-902.
    • Zhou JB., et al. Identification of a novel gene fusion RNF213‑SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep. 2013 Feb;7(2):591-7.
    • Jessica Hoppstdter., etc. Glucocorticoid‐induced leucine zipper is downregulated in human alveolar macrophages upon Toll‐like receptor activation. Eur J Immunol. 2012 May;42(5):1282-93.


    Related Products
    Cat. No.
    Name
    Size   
    Price
    Selected
    D0056
    0.500 ml
    $25
    E00008
    1000 U
    $60
    E00043
    1000 U
    $60
    E00101
    1 PK
    $78
    1
    Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
    2
    PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
    3
    Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
    4
    Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.