molecular biology services
Cat. No.
1000 U
50000 U (50 x 1000 U)
1000 U

Taq DNA Polymerase IS a thermostable DNA Polymerase isolated FROM an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase IS the most common polymerase used FOR PCR.

  • Note: This product IS supplied WITH 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see ApplicationsThe applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing

    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    QC Tests
    PCR* performance, activity, nuclease

    Lane Taq Unit
    Brand A
    2 0.25
    3 0.5
    Brand B
    5 0.25
    6 0.5
    8 0.25
    9 0.5

    Store the product at -20°C.

    * The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.



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    • Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol. 2013 Mar;27(3):511 - 523.
    • Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One. 2013 Jan;8(1):e50627.
    • Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A. 2013 Mar;110(10):3943 - 3948.
    • Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One. 2013 May;6(5):e62394.
    • Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb). 2013 Jul;111(1):16-22.
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    • Zhou JB., et al. Identification of a novel gene fusion RNF213‑SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep. 2013 Feb;7(2):591-7.
    • Jessica Hoppstdter., etc. Glucocorticoid‐induced leucine zipper is downregulated in human alveolar macrophages upon Toll‐like receptor activation. Eur J Immunol. 2012 May;42(5):1282-93.

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