molecular biology services
Cat. No.
Name
Size   
Price
Selected
E00043
1000 U
$60
E00007-50000
50000 U (50 x 1000 U)
$2,700
E00007-1000
1000 U
$60

Taq DNA Polymerase IS a thermostable DNA Polymerase isolated FROM an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase IS the most common polymerase used FOR PCR.

  • Note: This product IS supplied WITH 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).
  • Applications
    The applications of Taq DNA Polymerase include the following:
    • PCR*
    • 3'A-tailing of blunt ends
    • Primer extension
    • DNA sequencing


    Formulation
    GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
    Unit Definition
    One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100
    Buffer. This buffer is optimized for use with 200 µM dNTPs.
    Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
    Concentration:
    Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    QC Tests
    PCR* performance, activity, nuclease


    Lane Taq Unit
    1Leading
    Brand A
    0.1
    2 0.25
    3 0.5
    4Leading
    Brand B
    0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5

    Storage
    Store the product at -20°C.

    * The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.


    Document


    References

    • Gerard Crudden, Rachel E. Chitti, and Rolf J Craven. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J. Pharmacol. Exp. Ther. Oct 2005;
    • Zeyu Xin, Yihong Zhao, and Zhi-Liang Zheng. Transcriptome Analysis Reveals Specific Modulation of Abscisic Acid Signaling by ROP10 Small GTPase in Arabidopsis. Plant Physiol. Oct 2005; 139(3): 1350-1365
    • Wang X., et al. Salicylic Acid Regulates Plasmodesmata Closure during Innate Immune Responses in Arabidopsis. Plant Cell. 2013 Jun;25(6):2315-29.
    • Ruth B. Phillips., et al. Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome. Int J Genomics. 2013 Mar;261730.
    • FrÍas M., et al. The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif in the protein surface. Mol Plant Pathol. 2013 Qct;12097.
    • Y Acanda., et al. EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine. Plant Cell Rep. 2013 Dec.
    • Firlej A., et al. A Multi-Approach Study to Delineate Interactions Between Carabid Beetles and Soybean Aphids. Environ Entomol. 2013 Feb;42(1):89 - 96.
    • Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol. 2013 Mar;27(3):511 - 523.
    • Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One. 2013 Jan;8(1):e50627.
    • Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A. 2013 Mar;110(10):3943 - 3948.
    • Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One. 2013 May;6(5):e62394.
    • Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb). 2013 Jul;111(1):16-22.
    • Carol D. von Dohlen., et al. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid (Adelges tsugae)(Hemiptera: Adelgidae) from its native and introduced range. Environ Microbiol. 2013 Jul;15(7):2043-62.
    • Gandhirajan RK., et al. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation. J Clin Invest. 2013 Feb;123(2):887-902.
    • Zhou JB., et al. Identification of a novel gene fusion RNF213‑SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep. 2013 Feb;7(2):591-7.
    • Jessica Hoppstdter., etc. Glucocorticoid‐induced leucine zipper is downregulated in human alveolar macrophages upon Toll‐like receptor activation. Eur J Immunol. 2012 May;42(5):1282-93.


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