ApplicationsThe applications of Green Taq DNA Polymerase include the following:
- PCR*(For simple templates, this enzyme can be optimized for amplification of PCR products up to 10 kb; However, for complex templates, this enzyme can be used for amplification of PCR products up to 3 kb.)
- 3'A-tailing of blunt ends (T/A-cloning)
- Primer extension
- DNA labeling reactions
FormulationThe polymerase is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction. It can be stable for six months if stored at 4°C or for up to one month without significant loss of activity at ambient temperature.
Unit DefinitionOne unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
PCR Reaction Buffer (with Mg2+) 100 mM Tris-HCl, 15 mM MgCl2, 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C).
Enzyme Storage Buffer 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C)
StorageThis product is shipped at ambient temperatures for several weeks. Green Taq DNA Polymerase will remain stable for at least six months if stored at 4°C and for at least one year if stored at -20°C.
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