eStain® 2.0 Protein Staining System Next generation of protein gel staining technology
 | · Faster - complete protein gel staining in 7 minutes or less
· Easier - hit the run button and walk away
· Cleaner - no additional buffers required
· Safer - unique formulation without methanol |
| | Innovative electric staining system for faster and more convenient protein gel staining | | | | | | | The eStain® 2.0 Protein Staining System, applying GenScript's patent-pending electric staining technology (Figure 1), enables you to quickly, reliably and efficiently stain protein gels with Coomassie blue dye within 7 minutes or less. As a user-friendly unit, the eStain® 2.0 Protein Staining Device uses disposable eStain® 2.0 Protein Staining Pads containing Coomassie blue dye in proprietary electrode buffers, offering easy and convenient procedures without the need for additional buffers. |  | | Figure 1. How eStain® 2.0 electric staining works. A certain voltage is applied to the graphite anode and titanium cathode of the eStain® 2.0 Device, driving negatively charged Coomassie blue dye reagents into the gel matrix between the eStain® Pads to stain the proteins and the free staining reagents out of the gel matrix to destain the gel. |
| | Shortened workflow increases your productivity | | | | | | | The eStain® 2.0 Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1). The protein gel staining is finished so fast that you can see the staining results immediately after electrophoresis. | | Table 1 - Faster protein gel staining with the eStain® 2.0 |  | Note: Competitors 3 also uses a microwave protocol which takes 15 to 30 minutes but often gives high blue background and requires extended destaining.
| | Optimized self-contained system offers easy and convenient procedures | | | | | | The eStain® 2.0 Protein Staining System is created as a self-contained system. The ready-to-use eStain® Protein Staining Pads contain the required electrode buffers with Coomassie blue dye incorporated in the cathode buffer. No additional solutions and buffers are required. Furthermore, the proprietary formulation does not contain methanol, therefore, the used eStain® Pads need no special treatment before disposal. With eStain® 2.0 System, all you need to do is to simply assemble the eStain® Pads together with the pre-run gel, then press the run button (Figure 2).
Figure 2. Schematic workflow of eStain® 2.0 Protein Staining System. |
| | | | | In addition to ease and convenience, the eStain® 2.0 Protein Staining System provides high detection sensitivity comparable to that of traditional tri-step method (Figure 3). In the end, you'll see sharp protein bands out of clear background. |  | | Figure 3. High staining efficiency achieved using the eStain® 2.0 System. 7 ul New England Biolabs® Protein Ladder (P7703S) were loaded in each lane and separated on 4-20% Express PAGE Gels (Bis-Tris gel). The gels were stained either by eStain® 2.0 System (L) or by traditional Coomassie blue staining method (R) with CBB R-250.
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| | Suitable for various types of mini gels | | | | | | | The eStain® 2.0 Protein Staining System is designed to work with multiple types of mini polyacrylamide gels, including Tris-Glycine, Bis-Tris, Tris-Tricine, and Tris-Acetate gels, etc. |
| | | | Available Video | | Watch the video and see the estain® 2.0 System in action | | | Ordering Information
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