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ExpressTM PAGE Gels

GenScript ExpressTM PAGE Gels

The ExpressTM PAGE Gels perfect protein analysis with full range of precast polyacrylamide gels. GenScript's proprietary techniques, give ExpressTM PAGE Gels better speed, higher resolution, and higher transfer efficiency than other leading gels.

  • Easy to use: This product is simple to set up, without cumbersome combs or tapes.
  • Long Shelf Life: ExpressTM Page Gels last up to one year if stored at 4°C.
  • High Resolution: Using unique running buffer to produce excellent separation and high-resolution bands.
  • Fast Electrophoresis: 30- to 45- minute runtime
  • High Transfer Efficiency: Complete protein transfer in 30 min using semi-dry technique.

  • Gel Selection Guide
  • Related Resources
  • Example

Note: GenScript ExpressTM PAGE gels are formulated using 1X Tris-MOPS-SDS buffer (PH 6.8). For best results, please use GenScript running buffer (Cat. No. M00138). This buffer will ensure excellent separation and high-resolution bands. Otherwise, please prepare buffers according to our protocol.
* Free samples available.

Cat.No. Name Size Price Quatity
MB01015 5X Sample Buffer 5 ml $24.50
M00138* Express Gel Running Buffer Powder 1 Box
(5/PK)		 $38.00
M00139* Transfer Bufffer Powder 1 Box
(10/PK)		 $58.00
*To make 1000 ml 1X Running Buffer or Transfer Bufffer per package



Cat. No. Separation Range % Acrylamide Wells Vol. Size Price Quantity
MG420W10* 205 - 6.5 kDa 4-20% 10 50 μl 1 Box of 10 $68.00
MG420W12 12 30 μl 1 Box of 10 $68.00
MG420W15 15 25 μl 1 Box of 10 $68.00
MG816W10 205 - 14 kDa 8-16% 10 50 μl 1 Box of 10 $68.00
MG816W12 12 30 μl 1 Box of 10 $68.00
MG816W15 15 25 μl 1 Box of 10 $68.00
MG012W10 12% 10 50 μl 1 Box of 10 $68.00
MG012W12 12 30 μl 1 Box of 10 $68.00
MG012W15 15 25 μl 1 Box of 10 $68.00
MG010W10 205 - 24 kDa 10% 10 50 μl 1 Box of 10 $68.00
MG010W12 12 30 μl 1 Box of 10 $68.00
MG010W15 15 25 μl 1 Box of 10 $68.00
MG008W10 205 - 45 kDa 8% 10 50 μl 1 Box of 10 $68.00
MG008W12 12 30 μl 1 Box of 10 $68.00
MG008W15 15 25 μl 1 Box of 10 $68.00


Protein Migration Table:

The Protein Migration Table as following can also help you to choose the appropriate gels for your protein electrophoresis. The data of this table is from gels run at 140 volts for 45 min. At 140 volts, the Bromophenol Blue band will reach the bottom of the gels in about 30 min.

protein migration

Recipe of 5X Sample Buffer*:
SDS 1.0 g
Glycerol 5.0 ml
Bromophenol Blue 25 mg
Tris Base 150 mg (adjust the PH to 6.8)
2-Mercappethanol 1.0 ml
Deionized Water To 10 ml

Recipe of 10X Running Buffer* and 20X Transfer Buffer*:
  10X Running Buffer (Poweder) 20X Transfer Buffer (Poweder)
Tris Base 60.6 g 60.0 g
Bicine   81.6 g
MOPS 104.6 g  
SDS 10.0 g  
EDTA 3.0 g  
Deionized Water To 1000 ml 1000 ml
Note To make 1L of 1X transfer buffer: mix 50 ml of 20X Transfer Buffer, 100 ml of methanol or ethanol and 850 ml of deionized water.

*Buffer can be ordered separately here


Cat.No. Name Size Price Quatity
MB01015 5X Sample Buffer 5 ml $24.50
M00138* Express Gel Running Buffer Powder 1 Box
(5/PK)		 $38.00
M00139* Transfer Bufffer Powder 1 Box
(10/PK)		 $58.00
*To make 1000 ml 1X Running Buffer or Transfer Bufffer per package

Compatible Gel Tanks:

Express Gels are compatible with the following Gel Tanks:

  • GradiGel Mini 4-Cell
  • IBI Universal Protein System
  • EC 4-Cell
  • Hoefer Tall Mighty Small™ (SE 280)
  • Hoefer Mighty Small™(SE 260/SE 250)
  • Daiichi Mini 2-Gel & 6-Gel
  • Owl Road Runner, Penguin
  • Novex XCell I and II™ and Surelock™
  • Bio-Rad Mini-PROTEAN™ II & 3
  • Owl Single Sided Vertical System

Download Documents: TM0210
Examples with ExpressTM Precast Gels

Figure 1. Proteins ranging from 205 kDa to 6.5 kDa would be resolved best on a 4-20% ExpressTM PAGE gels as following performance:

Precast Gels for 4-20% detection

Lane 1.2.3.10: Smart Broad-Range Protein Standard (GenScript, MM0906)
Lane 4.5.6: Smart Protein Standard (Middle-Range)
(GenScript, MM0900)
Lane 7.8.9: EasyWestern Protein Standard
(GenScript, MM0908)
  ExpressTM Precast Gel
Cell Concentration 4-20%
Gel Running 140 volts 45 min
Semi-Dry Protein Transfer 20 volts 30 min

Figure 2. Comparison of ExpressTM Precast Gels with other Precast Gels for Western Blot detection of EasyWestern Protein Marker. After electrophoresis, the EasyWestern Protein Marker were transferred to nitrocellulose membrane and then detected using ONE-HOUR WesternTM His-Tag Western Detection Kit L00212. This experiment was repeated by using a 4-20% Precast Gel (Brand A) from company A.

Comapred with Other Product , Express Gels needed less time and gave better results, bigger proteins (in this case, 75 kD and 100 kD His-tagged proteins) were transferred more efficiently from ExpressTM Gels.

Lane 1.2.3.4.5.6: EasyWestern Protein Marker (Genscript, Cat. No.MM0908)
Lane 1: 10 μl   Lane 2: 5.0 μl  Lane 3: 2.5 μl
Lane 4: 1.3 μl  Lane 5: 0.6 μl  Lane 6: 0.3 μl
  ExpressTM Precast Gel Brand A Precast Gel
Cell Concentration 4-20% 4-20%
Gel Running 140 volts
45 min 		140 volts
45 min
Semi-Dry Protein Transfer 20 volts
30 min 		30 volts
45 min