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GenScript ExpressTM PAGE Gels


GenScript ExpressTM PAGE Gels are precast polyacrylamide gels for protein gel electrophoresis. Using proprietary techniques, ExpressTM PAGE Gels are made to have long shelf life, high resolution, fast electrophoresis, high transfer efficiency. ExpressTM PAGE Gels are better choices for protein analysis with the following major breakthroughs:
  • Easy to use - no comb or tapes to be removed, very simple to set up.
  • Long shelf life - up to two years (stored at 4°C).
  • High resolution - using unique running buffer to produce excellent separation and high-resolution bands.
  • Fast electrophoresis - 30 to 45 minute run time for quick results.
  • High transfer efficiency - complete protein transfer in 30 min using semi-dry technique.

Gel Selection Guide:

Cat. No. %Acryl- amide WellsWell Vol.Separation RangeSizePriceQuantity
MG008W10 8% 10 50 μl 205 - 45 kDa 1 Box of 10 $102.50
MG010W10 10% 10 50 μl 205 - 24 kDa 1 Box of 10 $102.50
MG012W10 12% 10 50 μl 205 - 14 kDa 1 Box of 10 $102.50
MG420W10 4-20% 10 50 μl 205 - 6.5 kDa 1 Box of 10 $102.50
MG816W10 8-16% 10 50 μl 205 - 14 kDa 1 Box of 10 $102.50
 
MG008W12 8% 12 30 μl 205 - 45 kDa 1 Box of 10 $102.50
MG010W12 10% 12 30 μl 205 - 24 kDa 1 Box of 10 $102.50
MG012W12 12% 12 30 μl 205 - 14 kDa 1 Box of 10 $102.50
MG420W12 4-20% 12 30 μl 205 - 6.5 kDa 1 Box of 10 $102.50
MG816W12 8-16% 12 30 μl 205 - 14 kDa 1 Box of 10 $102.50
 
MG008W15 8% 15 25 μl 205 - 45 kDa 1 Box of 10 $102.50
MG010W15 10% 15 25 μl 205 - 24 kDa 1 Box of 10 $102.50
MG012W15 12% 15 25 μl 205 - 14 kDa 1 Box of 10 $102.50
MG420W15 4-20% 15 25 μl 205 - 6.5 kDa 1 Box of 10 $102.50
MG816W15 8-16% 15 25 μl 205 - 14 kDa 1 Box of 10 $102.50




Protein Migration Table:
This protein migration table can also help you to choose the appropriate gels for your protein electrophoresis. The data of this table is from gels run at 140 volts for 45 min. At 140 volts, the Bromophenol Blue band will reach the bottom of the gels in about 30 min.


Compatible Gel Tanks:
Express Gels are compatible with the following Gel Tanks:
  • GradiGel Mini 4-Cell
  • IBI Universal Protein System
  • EC 4-Cell
  • Hoefer Tall Mighty SmallTM (SE 280)
  • Hoefer Mighty SmallTM (SE 260/SE 250)
  • Daiichi Mini 2-Gel & 6-Gel
  • Owl Road Runner, Penguin
  • Novex XCell I and IITM and SurelockTM
  • Bio-Rad Mini-PROTEANTM II & 3
  • Owl Single Sided Vertical System

Related Products:

ProductCat. No.SizePriceQuantity
5X Sample Buffer MB01015 5 ml $24.50
10X Running Buffer MB01012 500 ml $44.50
20X Transfer Buffer MB01014 500 ml $60.50




Recipe of 5X Sample Buffer:

5X Sample Buffer:
SDS 1.0 g
Glycerol 5.0 ml
Bromophenol Blue 25 mg
Tris base 150 mg (adjust the pH to 6.8)
2-Mercapoethanol 1.0 ml
Deionized water to 10 ml


Recipe of 10X Running Buffer and 20X Transfer Buffer:


10X Running Buffer 20X Transfer Buffer*
Tris base 121 g 60.0 g
Bicine   81.6 g
HEPES 238 g  
SDS 10.0 g  
Deionized water to 1000 ml 1000 ml
*To make 1L of 1X transfer buffer: mix 50 ml of 20X Transfer Buffer, 100 ml of methanol or ethanol and 850 ml of deionized water.

Storage:
Store all the Gels and Buffers at 4 - 8°C.

Examples Using the Gels:
Comparison of ExpressTM Precast Gels with other Precast Gels.
ExpressTM PAGE Gels were compared with precast gels (Brand A) from company A for Western Blot detection of His-tagged proteins.
  ExpressTM Precast Gel Brand A Precast Gel
Gel Concentration 4 - 20% 4 - 20%
Gel Running 140 volts
45 min		 140 volts
80 min
Semi-Dry Protein Transfer 20 volts
30 min		 30 volts
45 min
Figure 1. Comparison of ExpressTM Precast Gels with other Precast Gels for Western Blot detection of His-tagged proteins. 10 μl, 5.0 μl, 2.5 μl, 1.3 μl and 0.6 μl of the 6xHis Protein Ladder (Qiagen, Cat. No. 34705, reconstituted in 250 μl 1X SDS-PAGE sample buffer as suggested by Qiagen) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, and Lane 5 of a 4 - 20% ExpressTM PAGE Gel, respectively. After electrophoresis, the His-tagged proteins were transferred to nitrocellulose membrane and then detected using One-StepTM His-Tag Western Detection Kit L00212. This experiment was repeated by using a 4 - 20% Precast Gel (Brand A) from company A.

Comapred with Brand A precast gels, ExpressTM PAGE Gels needed less time and gave better results, bigger proteins (in this case, 75 kD and 100 kD His-tagged proteins) were transferred more efficiently from ExpressTM PAGE Gels.



Technical Manual: