Protein Migration Table:
The Protein Migration Table as following can also help you to choose the appropriate gels for your protein electrophoresis. The data of this table is from gels run at 140 volts for 45 min. At 140 volts, the Bromophenol Blue band will reach the bottom of the gels in about 30 min.

Recipe of 5X Sample Buffer*:
| SDS |
1.0 g |
| Glycerol |
5.0 ml |
| Bromophenol Blue |
25 mg |
| Tris Base |
150 mg (adjust the PH to 6.8) |
| 2-Mercappethanol |
1.0 ml |
| Deionized Water To |
10 ml |
Recipe of 10X Running Buffer* and 20X Transfer Buffer*:
| |
10X Running Buffer (Poweder) |
20X Transfer Buffer (Poweder) |
| Tris Base |
60.6 g |
60.0 g |
| Bicine |
|
81.6 g |
| MOPS |
104.6 g |
|
| SDS |
10.0 g |
|
| EDTA |
3.0 g |
|
| Deionized Water To |
1000 ml |
1000 ml |
| Note |
To make 1L of 1X transfer buffer: mix 50 ml of 20X Transfer Buffer, 100 ml of methanol or ethanol and 850 ml of deionized water. |
*Buffer can be ordered separately here
Compatible Gel Tanks:Express Gels are compatible with the following Gel Tanks:
- GradiGel Mini 4-Cell
- IBI Universal Protein System
- EC 4-Cell
- Hoefer Tall Mighty Small™ (SE 280)
- Hoefer Mighty Small™(SE 260/SE 250)
- Daiichi Mini 2-Gel & 6-Gel
- Owl Road Runner, Penguin
- Novex XCell I and II™ and Surelock™
- Bio-Rad Mini-PROTEAN™ II & 3
- Owl Single Sided Vertical System
Download Documents:
Examples with ExpressTM Precast Gels
Figure 1. Proteins ranging from 205 kDa to 6.5 kDa would be resolved best on a 4-20% ExpressTM PAGE gels as following performance:
 |
Lane 1.2.3.10: Smart Broad-Range Protein Standard (GenScript, MM0906) Lane 4.5.6: Smart Protein Standard (Middle-Range) (GenScript, MM0900) Lane 7.8.9: EasyWestern Protein Standard (GenScript, MM0908) |
| |
ExpressTM Precast Gel |
| Cell Concentration |
4-20% |
| Gel Running |
140 volts 45 min |
| Semi-Dry Protein Transfer |
20 volts 30 min |
|
Figure 2. Comparison of ExpressTM Precast Gels with other Precast Gels for Western Blot detection of EasyWestern Protein Marker. After electrophoresis, the EasyWestern Protein Marker were transferred to nitrocellulose membrane and then detected using ONE-HOUR WesternTM His-Tag Western Detection Kit L00212. This experiment was repeated by using a 4-20% Precast Gel (Brand A) from company A.
Comapred with Other Product , Express Gels needed less time and gave better results, bigger proteins (in this case, 75 kD and 100 kD His-tagged proteins) were transferred more efficiently from ExpressTM Gels.
 |
|
|
|
Lane 1.2.3.4.5.6: EasyWestern Protein Marker (Genscript, Cat. No.MM0908)
|
| Lane 1: 10 μl Lane 2: 5.0 μl Lane 3: 2.5 μl |
| Lane 4: 1.3 μl Lane 5: 0.6 μl Lane 6: 0.3 μl |
| |
ExpressTM Precast Gel |
Brand A Precast Gel |
| Cell
Concentration |
4-20% |
4-20% |
| Gel Running |
140 volts
45 min |
140 volts
45 min |
| Semi-Dry Protein
Transfer |
20 volts
30 min |
30 volts
45
min |
|