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Catalog Products » Protein Isolation and Analysis » Protein Electrophoresis » ExpressPlus™ PAGE Gels, 10×8 series
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ExpressPlus™ PAGE Gels
Compatible with more gel tanks!

· Large Loading Volume: Twice as large as the common gel on the market
· Long Shelf Life - Up to 12 months if stored at 2-8°C.
· High Reproducibility: Consistent performance from gel to gel.

  10×8 series  

   

GenScript ExpressPlus™ PAGE Gels have been upgraded to be compatible with more gel tanks. More researchers can experience precast gels with a shorter running time, larger loading volume, higher transfer efficiency at affordable prices. The ExpressPlus™ PAGE Gels are cast in a weak acidic pH environment that minimizes the hydrolysis of polyacrylamide and results in extra gel stability and superior band resolution.


  How to choose ExpressPlus? PAGE Gels? Click here to view more  

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No additional purchase of running buffer needed!

  • 1 Unit=20 gels+10 Packs of free-mixed MOPS buffer, 10 free packs of MOPS buffer, valued at $50!
  • Pricing valid in North America only
Resources

10x8 gel ordering list


Cat. No.
Product Name
Size
Price
Quantity
MB01015 5X Sample Buffer
5 ml
$24.00
M00138 Tris-MOPS-SDS Running Buffer Powder
1 Box (5/PK)
$25.00
M00139 Transfer Buffer Powder
1 Box (10/PK)
$58.00
 


* Price valid on GenScript ExpressPlus? PAGE Gel orders for the US and Canada only. Customers ordering from other countries please contact Product@GenScript.com for the latest pricing information in your region.

** GenScript ExpressPlus™ PAGE Gels are formulated using 1x Bis-Tris buffer (pH 6.8). For best results, please use GenScript running buffer (Cat. No. M00138) for gel running, and GenScript transfer buffer (Cat. No. M00139) for protein transfer after running the ExpressPlus™ PAGE gels. Otherwise, please prepare buffers according to our protocol.

Protein Migration Table

The Protein Migration Table below helps you choosing the appropriate gels for protein electrophoresis. The data of this table is built on electrophoresis at 10 volts for 55 min using ExpressPlus™ PAGE gels.

10x8 Gel

precast gel
  • precast gel

    Figure 1. Coomassie blue staining result using 12 well ExpressPlus 4-20% PAGE gel (M42012)


    Lane 1, 2: GenScript Broad Protein maker Ⅱ
    Lane 3, 4: E.Coli lysate
    Lane 5: TaKaRa Protein Molecular Weight Marker (Broad) Code NO. 3452
    Lane 6, 7, 8, 9, 10: GenScript PAGE-MASTER Protein Standard (M00516), each sample of lane 6, 7, 8, 9 and 10 contains: 10 μl, 5 μl, 2.5 μl, 1.25 μl, 0.63 μl.
    Lane 11, 12: GenScript PAGE-MASTER Protein Standard Plus (MM1397-500)

  • precast gel

    Figure 2. Chemiluminescent Western blot detection of Protein G(GenScript, Z02007) on GenScript ExpressPlus™ PAGE Gel.


    EasyWestern Protein Standard (GenScript, MM0908), was separated on a 4-20% ExpressPlus™ PAGE Gel and transferred to nitrocellulose membrane. The blot was blocked overnight with 10% fat-free milk, detected with a Anti-RABBIT IgG (H&L) (GOAT) Antibody (Rockland, 611-132-122). Scanned by odyssey(LI-COR). Lane 1-5 contained Protein G at 200ng, 100ng, 50ng, 10ng and 5ng, Lane 6-10 contained EasyWestern Protein Standard at 4μl, 2μl, 1μl, 0.5μl, 0.25μl, respectively.

  • precast gel

    Figure 3. Proteins were separated on a 12-well, 12% ExpressPlus™ PAGE Gel (M01212) and then stained using the eStain® Protein Staining System (R-250).

    Lane 1, 2, 6, 7, 11, 12: 6 μl E.Coli lysate
    Lane 3, 8: 5 μl NEB Protein Standard (P7703S).
    Lane 4, 9: 5 μl GenScript PAGE-Master Protein
    Standard (M00516)
    Lane 5, 10: Life Tech. Mark12™ Unstained Standard (LC5677)

  • precast gel

    Figure 4. Western Blots: Single Percentage Gels 7.5% & 8%

      Supplier Company BR GenScript (Cat.no. M00812)
      Gel % 7.5% 8%
    Lane 1 0.5uL Plasma 3,178 3,677
    Lane 2 0.2uL Plasma 1,426 2,154
    Lane 3 1000ng Transferrin 1,408 3,208
    Lane 4 100ng Transferrin 180 387
    Lane 5 10ng Transferrin 155 143
      Bkg 155 163

FAQ

  1. How long does it take to run a gel?
    It depends on the concentration of the gel. Usually it takes about 50 minutes at 140V. Shorter running times can be achieved if a higher voltage is used, but higher than 180V is not recommended.
  2. Why do some parts of the gel become sticky following protein transfer?
    This is caused by the concentrated polyacrylamide on the top part of the gel. It will help if this part of the gel is cut and removed before transfer.
  3. Will overnight destaining affect the resolution of the band?
    It depends. We suggest using a destaining solution consisting of 15% methanol and 10% acetic acid in DI water for overnight destaining.
  4. How should I adjust the staining time on the eStain® 2.0 when using different types of ExpressPlus™ PAGE gels?
    Usually, an additional 1-2 minutes is recommended for gels that have a higher SDS concentration. We also recommend that users optimize conditions for their specific gel type.
  5. Is this gel suitable for running both SDS-PAGE proteins and Native-PAGE proteins?
    It is. However, running native-PAGE proteins on ExpressPlus™ PAGE gels takes much longer (~1.5h in total) than SDS-PAGE proteins. We recommend optimizing your conditions (time and voltage) before running native-PAGE proteins for an experiment.
  6. What running tanks are ExpressPlus™ PAGE gels compatible with?
    Any tank that is designed to run 10cm x 8cm (length x width) precast gels.
  7. Is this gel stable at room temperature?
    Yes. ExpressPlus™ PAGE gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.
  8. What running buffer should I use to run the gel?
    We recommend MOPS for normal use, and MES for small proteins. Tris-glycine buffer is NOT compatible with ExpressPlus™ PAGE gels.

Trouble shooting

Problem
Possible Cause
Suggestion
Distorted protein bands
Air bubbles in the sample wells, or between gel and cassette
Use a syringe or other appropriate tools to flush the sample wells thoroughly with running buffer
Indicator strip partly turns yellow
Buffer goes into gel through broken cassette
Use compatible gel tanks, make sure the cassette is not cracked
pH value decreased
Make new running buffer with deionized water
Streaking
Poorly soluble or weakly charged particles (such as carbohydrates) in sample
Heat sample in the presence of SDS, centrifuge sample and load the supernatant
Electrophoresis time is too long
Seal is not removed
Peel off the seal at bottom of cassette before loading
Incorrect running condition
Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis
Running without a plastic cushion in Bolt™ tank
Put the cushion into the bottom of Bolt™ tank before insert the gel cassette to run
Running buffer leaking from Bolt™ tank during electrophoresis
Adjust the position of the cassette edge with the gasket of Bolt™ tank to avoid buffer leaking
Bands are difficult to distinguish
Incorrect gel percentage
Use the protein migration table to choose the appropriate gels
Sample overloading
Reduce sample amount, do not load more than 50 μg (total protein) in a single well
Insufficient SDS in loading buffer
Enhance SDS in loading buffer when preparing your sample
Insufficient buffer to keep tank cool
For best results, the buffer in the outer tank should be approximately level with the bottom of the sample wells
Sample spreading across the gel
Sample contains too much salt
Reduce salt by dialysis or ultra-filtration
Ambiguous band at the same position of indicator strip
Ion disturbance in gel (higher chance when analyzing small proteins)
Use MES running buffer
Run the gel with longer running time or ignore the band
The voltage cannot reach set value
Leaking between the inner and outer tank during electrophoresis
Use compatible gel tank
Excess salt in the sample
Reduce salt by dialysis or ultra-filtration
Lots of air bubbles between the gel and the cassette
Running buffer is hot after electrophoresis
Run the gel at 4°C
Increase the running buffer amount in outer tank
The sample volume doesn't reach the maximum loading volume of the sample well
Not careful and slow enough when loading the protein sample
Be careful and slow down when loading sample
Floating gels in the loading well
Improper removal of the comb
Remove the comb vertically and slowly
 
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