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Custom Monoclonal Antibody Production Services

Custom Monoclonal Antibody Production Services
With the FASTEST turnaround times in the industry

GenScript is a leader in custom monoclonal antibody production.  Our 10-year track record of generating thousands of successful custom monoclonal antibodies ensures we can develop high-specificity, high-affinity custom antibodies for your unique applications. We offer a full suite of all-inclusive fast monoclonal antibody production packages with unparalleled guarantees, yet without the standard set-up fees.  Each MonoExpress™ custom monoclonal antibody package delivers specific hybridoma in just 45 days with ELISA guarantee. In addition, our popular MonoExpress™ Gold package guarantees positive Western blot results using immunogen protein or there is no charge to you.

MonoExpress™ custom monoclonal antibody production services allow you to choose either peptide or recombinant protein as antigen. You simply supply the protein sequence and we will deliver your specific hybridoma within 45 days. MonoExpress™ Silver and Bronze custom monoclonal antibody production packages use peptide antigen and each starts with peptide antigen design using our proprietary OptimumAntigen™ design tool, followed by peptide synthesis, mouse or rat immunization, all the way to final specific hybridoma screening and selection. MonoExpress™ Gold and Basic packages use protein antigen: the Gold package includes protein antigen production, while the Basic package requires you to provide your own protein antigen.  In addition to the delivery of specific hybridomas, both packages also deliver 2 mg affinity-purified antibody with Western Blot guarantee.

If you have special requirements for your monoclonal antibody production, please check out our fully Customized Monoclonal Antibody Services.

Key Features

  • Optimized immunization: Our advanced technologies - OptimumAntigen™ design tool, our proprietary immunization technology, and new GANP mouse – increase specificity and affinity of antibodies.
  • Certified facility: AAALAC International accreditation and OLAW certification demonstrate our commitment to responsible animal care and use.
  • Guaranteed results: Western blot and ELISA titer guarantee.
  • Fast turnaround time: We deliver antibodies 4 months faster than traditional immunization protocols, specific hybridomas generated in 45 days and delivery of specific custom antibody in as little as 6 weeks.

  • MonoExpress™ Package Summary
  • MonoExpress™ Package Details
  • Selection Guide
  • Publications using MonoExpress™
  • Experimental Data

Why choose MonoExpress™ custom monoclonal antibody services?

Using recombinant protein to make antibody
Recombinant Protein as Antigen
Up to 5 antibody-producing hybridoma
Deliver up to 5 hybridoma
Western blot positive for immunogen guaranteed At least 1 clone Western Blot positive and ELISA titer of ≥ 1:64,000 guaranteed
Using 2 peptides to make antibodies
2 Peptide Antigens
4 antibody-producing hybridoma
Deliver 4 hybridoma
Western blot detection of native protein over 85% 4 supernatants for the first deliverable and ELISA positive for immunogen guaranteed
Using 1 peptide to make antibody
1 Peptide Antigens
2 -5 antibody-producing hybridoma
Deliver 2-5 hybridoma
Hundreds of successful cases of Western blot detection of native protein 2-5 supernatants for the first deliverable and ELISA positive for immunogen guaranteed
Using customer's protein antigen
Up to 5 antibody-producing hybridoma
Western blot positive for immunogen guaranteed Western blot positive for the immunogen and ELISA titer of ≥ 1:64,000 guaranteed

Details of MonoExpress™ custom monoclonal antibody services

Package Advantage and Guarantee Starting Material Deliverables First Delivery Timeline
MonoExpress™ Gold

Recombinant protein as antigen
(5 Balb/c mice or 3 rats)
SC1572P
Key Advantages:
  • Use of recombinant protein as immunogen increases successful antibody generation to over 95%
  • Customer can evaluate 10 supernatants to choose the 5 best hybridomas for their application
You provide: name, sequence (NCBI accession number if available) of target protein First Deliverable: 10 supernatants for evaluation**
11 weeks
Guaranteed: At least 1 clone positive for Western Blot*, ELISA titer≥1:64,000 We generate: 1 recombinant protein as immunogen Final Deliverables: Up to 5 hybridoma, 2mg purified antibody from one hybridoma, 200µg immunogen protein
MonoExpress™ Silver

2 peptides as antigen
(5 Balb/c mice or 3 rats per peptide)
SC1661
Key Advantage:
Use of 2 peptides to generate hybridomas increases successful antibody generation to over 85%.
You provide: name, sequence (NCBI accession number if available) of target protein First Deliverable: 4 supernatants (including at least one derived from each peptide antigen)
9 weeks
Guaranteed: 4 supernatants for 1st deliverable, ELISA positive for immunogen We generate: 2 peptide antigens to use as immunogen Final Deliverables: 4 hybridoma cell lines (at least 1 derived from each peptide antigen), 2mg unconjugated peptide
MonoExpress™
Bronze


1 peptide as antigen
(5 Balb/c mice or 3 rats)
SC1660
Key Advantage:
GenScript generates 1 peptide to use as immunogen with hundreds of successful antibodies generated
You provide: name, sequence (NCBI accession number if available) of target protein First Deliverable: Up to 5 supernatants
9 weeks
Guaranteed: 1-5 supernatants for 1st deliverable, ELISA positive for immunogen We generate: 1 peptide antigen to use as immunogen Final Deliverable: Up to 5 hybridoma cell lines, 2mg unconjugated peptide
MonoExpress™ Basic

You submit antigen
(5 Balb/c mice or 3 rats)
SC1572
Key Advantage:
Low cost and quick timeline with guaranteed WB positive for the immunogen
We use your provided protein antigen as the immunogen First Deliverable: Up to 5 supernatants
7 weeks
Guaranteed: ELISA ≥1:64,000, WB positive for the immunogen Final Deliverables: Up to 5 hybridoma cell lines, 2mg purified Ab from one hybridoma

Read Protocol and Timeline Details for MonoExpress™ packages.

If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody Services.

* MonoExpress™ Gold package guarantees that at least one clone will produce an antibody with positive results in WB using immunogen protein. GenScript will perform the Western Blot and include the positive results in the QC data sent to the client.
** If customer cancels the project after evaluating supernatants, the customer will only be charged for the first deliverables (supernatant), which is $3,000.
 GenScript provides free cell line storage service for 6 months from the date of cell freezing. If customers require continued storage service after the end of 6 month period, the charges are set at $10 per cell line per year.

What kind of antigen

What kind of antigen

Peer reviewed publications using our custom Monoclonal Antibody Services

Full List

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.

MonoExpress™ Protocol

MonoExpress™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:

  • Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
  • Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.

MonoExpress™ Protocol

A panel of monoclonal antibodies each having a unique binding specificity is shown:

  • Clone 1: Only recognized dual phosphorylated peptide.
  • Clone 2: Only recognized dual phosphorylated peptide.
  • Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
  • Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services

monoexpress antibody

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services.
Biacore antibody/antigen affinity analysis showed that the MonoExpress™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoExpress™ services display reasonably high affinities to their target antigens.

If above predefined packages don't meet your specific need, please choose our Custom Monoclonal Antibody Services for fully customizable monoclonal antibody production services.
For a general overview of our variety of monoclonal antibody services, click here.

Fastest Custom Monoclonal Antibody Services

Quotation and Ordering

Custom Monoclonal Antibody Production Services

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