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Monoclonal antibody production services
custom monoclonal antibody production service quotation

Custom Monoclonal Antibody Production Services

Promotion: FREE epitope binning! (SC1929)

Cost without promotion: $1,360
Minimizing non-specific binding. Better epitope diversity and clonal variability. Independent antibodies (binding to protein antigen via different epitopes) are included. Promotion applies to SC1717 and SC1711. Independent antibodies identified by epitope binning are guaranteed to be included in the first delivery of supernatants, further assisting your final choice of ideal clones. (Depending on the clients preference, the final chosen clones may or may not include independent antibody pairs).

GenScript has 12-year track record of custom monoclonal antibody production services, generating thousands of successful custom monoclonal antibodies with high-specificity and high-affinity. With our new MonoExpress™ Premium monoclonal antibody production services, you get to test 10 hybridoma supernatants and choose up to 5 clones that actually work for your own applications. No matter your application is Western Blot, IF/flow cytometry, IHC, ICC, IP/ChIP, Sandwich ELISA, or functional assays, we guarantee that at least one clone will work in your own experimental system. Read more »

MonoExpress™ Premium package uses protein as antigen, the gold standard when it comes to high-quality monoclonal antibody production. Protein antigens result in monoclonal antibodies against multiple conformational epitopes of the target protein, maximizing the chances your antibody will work for a variety of applications. To get started, simply provide your target protein sequence; we start with protein antigen production all the way to the production of application guaranteed monoclonal antibodies. MonoExpress™ Gold, Silver, and Bronze monoclonal antibody production services use peptides as antigens and all MonoExpress™ packages come with superior ELISA guarantees. Plus, you'll receive your antibody or hybridoma cell line 4 months faster than traditional immunization protocols.

Key Features of MonoExpress™ Custom Monoclonal Antibody Production Services

MonoExpress™ Custom Monoclonal Antibody Service Packages

Monoclonal antibody production using protein as antigen

Protein antigens are the elite choice when it comes to high-quality monoclonal antibody production. Their unique ability to elicit monoclonal antibodies that recognize the native confirmation of the target protein makes them a superior choice over other antigen strategies. Our popular application-guaranteed MonoExpress™ Premium monoclonal antibody production package exemplifies the high success rate of protein antigens by offering the unprecedented guarantee that at least one clone will work in your own experimental system.

Protein Antigen Packages
Guarantees
Starting Material
Immunization
Customer Screening
Deliverables
First Delivery
  • Application-positive for one clone*
  • ELISA ≥ 1:64,000
  • Protein antigen produced by GenScript
  • You provide: name, sequence (NCBI accession number if available) of target protein
5 Balb/c mice or 3 rats
10 ELISA(+) supernatants for evaluation in client's own experimental system*
  • Up to 5 hybridoma cell lines of customer's choice+
  • 2 mg purified mAb from one chosen hybridoma
  • 200 μg immunogen protein
11 weeks
  • WB positive for immunogen
  • ELISA ≥ 1:64,000
We use your provided protein antigen as the immunogen
5 Balb/c mice or 3 rats
5 ELISA(+) supernatants for evaluation in client's own experimental system*
  • Up to 5 hybridoma cell lines of customer's choice+
  • 2 mg purified mAb from one chosen hybridoma
6-7 weeks

+ Additional clones may be purchased at $399/clone.


Monoclonal antibody production using peptide as antigen

In cases where access to a target protein is limited or unavailable, but the amino acid sequence is known, peptide antigens offer a smart solution for monoclonal antibody production. Peptide antigens are also ideal for producing monoclonal antibodies against post-translational modification sites within a target protein.

Peptide Antigen Packages
Advantages and Guarantees
Starting Material
Immunization
Deliverables
Delivery Timeline
  • Guaranteed ELISA positive for immunogen
  • Use of 3 peptides to generate hybridomas increases successful Ab generation to > 95%.
  • GenScript generates peptides to use as immunogens
  • You provide: name, sequence (NCBI accession number if available) of target protein
15 Balb/c mice or 9 rats (5 Balb/c mice or 3 rats per peptide)
  • 6 hybridoma cell lines
  • (2 clones/ peptide)
  • 6 supernatants++ (5 ml/clone)
  • 2 mg unconjugated peptide
13 weeks
  • Guaranteed ELISA positive for immunogen
  • Use of 2 peptides to generate hybridomas increases successful Ab generation to > 85%.
10 Balb/c mice or 6 rats (5 Balb/c mice or 3 rats per peptide)
  • 4 hybridoma cell lines (2 clones/ peptide)
  • 4 supernatants++ (5 ml/clone)
  • 2 mg unconjugated peptide
  • Guaranteed ELISA positive for immunogen
  • GenScript generates 1 peptide to use as immunogen with hundreds of successful antibodies generated
5 Balb/c mice or 3 rats
  • 2 hybridoma cell lines (2 clones/ peptide)
  • 2 supernatants++ (5 ml/clone)
  • 2 mg unconjugated peptide

++ Purified mAb may be purchased at $399 for 2 mg purified mAb/clone.

What kind of antigen?

Peer reviewed publications using our custom Monoclonal Antibody Services

Full List

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.

MonoExpress™ Protocol

MonoExpress™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:

  • Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
  • Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.

MonoExpress™ Protocol

A panel of monoclonal antibodies each having a unique binding specificity is shown:

  • Clone 1: Only recognized dual phosphorylated peptide.
  • Clone 2: Only recognized dual phosphorylated peptide.
  • Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
  • Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services

monoexpress antibody

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services.
Biacore antibody/antigen affinity analysis showed that the MonoExpress™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoExpress™ services display reasonably high affinities to their target antigens.


*Read about our safety net policy

If a successful clone cannot be found, customer has the following options:

  1. Cancel the project after the first delivery of supernatants and only be charged $1,000 (applies to Premium (SC1711) and Basic (SC1717) packages).
  2. Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, customer may choose to cancel the project and only be charged $1,000 plus the cost of the additional 10 supernatants, for a total of $1,999 (applies to Premium (SC1711) package only).

For problematic antigens check out our DNA Immunization Service.

If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody Services

For a general overview of our variety of monoclonal antibody services, click here.



Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Fastest Custom Monoclonal Antibody Services

Quotation and Ordering

custom monoclonal antibody production service quotation

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.


 
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