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1. What is lyophilization?
2. What is the purpose and benefits of lyophilization?
3. What are the steps of freeze-drying?
4. When should reconstitution of the lyophilized antibody take place?
5. What buffer should be used for reconstitution?
6. Does the customer incur additional charge for lyophilization?
7. What other biologicals can lyophilization be applied to?
8. How long can lyophilized antibodies be preserved?
9. Are there any differences between lyophilized antibodies and antibodies in solution?
10. If insoluble material is observed in the reconstitution, will that affect the performance?
1. What is lyophilization?
Lyophilization is a freeze-drying method to preserve biologicals.
2. What are the purpose and benefits of lyophilization?
Lyophilization extends the shelf life of biologicals, preserving their chemical and biological properties. It involves less cumbersome storage and handling. Lyophilization reduces the temperature requirement for shipping and protects the biologicals' vitality. Compared with other methods, lyophilization dries biologicals at a lower temperature, reducing the damage to the product and retaining the molecular integrity. In addition, reconstitution of material is instant immediately following the addition of water.
3. What are the steps of freeze-drying?
Lyophilization involves three major steps. They are pretreatment, freezing, and drying which includes primary drying (sublimation) and secondary drying (desorption). The parameters involved in freezing and drying steps can be customized for optimum lyophilization results.
4. When should reconstitution of the lyophilized antibody take place?
The antibody should be stored lyophilized until use and reconstitution performed just before use.
5. What buffer should be used for reconstitution?
The lyophilized powder should be reconstituted by adding deionized or distilled water (or equivalent) with corresponding volume stated on the tube or report sheet.
6. Does the customer incur additional charge for lyophilization?
No, lyophilization is included in the corresponding antibody services.
7 .What other biologicals can lyophilization be applied to?
Other biologicals such as vaccines, purified proteins, peptides, and blood samples can be lyophilized.
8. How long can lyophilized antibodies be preserved?
Lyophilized antibodies are stable for at least one year without losing activity.
9. Are there any differences between lyophilized antibodies and antibodies in solution?
There is no difference between them. Lyophilization preserves the characteristics of the antibody in solution, making it suitable for long term storage. Antibodies in solution are not suitable for long term storage.
10. If insoluble material is observed in the reconstitution, will that affect the performance?
There will be no effect on the antibody performance.
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