Case Study—Co-expression of Protein Complexes
Purification of macromolecular complex subunits in large quantities and assembling them into functional entities are vital steps for successfully determining the structure and characterizing the functions of macromolecular complexes. Despite the fact that cases of isolation and structure determination of endogenous complexes have been demonstrated, great efforts still lie ahead to make this technology easily accessible. Recent endeavor on this subject suggests that co-expression of subunits within hosts of E.coli and insect cells, even for high-throughput projects, is becoming a promising solution.
GenScript has successfully co-expressed 3 subunits of A protein in E. coli system:
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Fig. A protein with His-tag was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane 1: A-His-αβγ target protein with His-tag |
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