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Baculovirus protein expression service

Case Studies - Mammalian Expression


GenScript recommends its mammalian expression services for cases in which the folding of the proteins is crucial to the customer's intended application and cases in which the internal machinery of E. coli is unable to express the protein with desired conformation and modification. Our proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.

1. Monoclonal Antibody Production

Fig. 1-1 SDS-PAGE analysis of A Antibody expressed in our mammalian expression system
Lane 1 and 2: reduced; Lane 3 and 4: non-reduced
Fig. 1-2 IEF analysis of A Antibody expressed in our mammalian expression system
 

2. Recombinant Protein Expression

Fig. 2-1 SDS-PAGE analysis of A Protein expressed in our mammalian expression system
Lane 1 and 2: reduced; Lane 3 and 4: non-reduced
Fig. 2-2 Western Blot analysis of A Protein expressed in our mammalian expression system
 

3. Membrane Protein

 
Fig. 3 Indirect Immunofluorescence detection of cell membrane protein expressed in our mammalian expression system