Applications of Gene Synthesis: High-Quality cDNA Synthesis
Replace costly PCR cloning
Synthesize cDNAs for which the corresponding mRNA sources are difficult to obtain
Synthesize predicted genes/cDNAs
Synthesize hard-to-clone genes or cDNAs
Full-length (or at least full-ORF) cDNAs are essential to the identification, cloning and characterization of functionally important genes. However, for many genes, isolating full-length cDNA is still a major technical challenge. Although cDNA libraries may be a highly efficient and convenient source of some cDNAs, the fragments derived from these libraries are usually very short and can only provide a little information. Covering a full-length cDNA requires several clones. This is especially important for rare species whose mRNA templates are hard to get and especially problematic for genes that have low abundance. Other factors, such as RNaseH activity and RNA secondary structure, can also affect the cloning of cDNA.
Gene synthesis is the most efficient means of obtaining high-quality cDNA or library constructs. With no need for splicing of short fragments, full-length cDNA can be produced in a very short amount of time. Rare-species cDNA can be synthesized using only the nucleotide sequence. Moreover, RNA secondary structure and repeat sequences can be optimized to boost final protein expression.
GenScript can provide you with any cDNA sequence using our powerful gene synthesis technology, boosting your research and freeing your schedule. GenScript guarantees its fast turnaround time.
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