OptimumGene™ - Codon Optimization |
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As one of the world's leading suppliers of gene synthesis services, GenScript has a proven record of dedication to innovation in our constant efforts to integrate new and updated technology into our gene expression repertoire. GenScript's OptimumGene™ Gene Design system is a proprietarily gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system. The OptimumGene™ algorithm takes into consideration of a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation.
- Advantages
- Algorithm
- Track Record
- Case Study
Advantages
 Significant increase in protein expression |
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Fruitful results in even the most difficult sequences:
GenScript's painstakingly crafted algorithms can optimize even highly repeated sequences, extremely long sequences, and sequences with large numbers of parameters. Our personal best is over 30, and we're willing to challenge it any time.
Comprehensive usage tables for optimization in any host:
GenScript boasts an extensive collection of proprietary codon usage tables, allowing you to use any host, any time in either basic research or industrial applications. Specifically, we have dependable codon tables for organisms, such as Pichia and Sf9, which are widely used in development procedures.
DNA vaccine design:
GenScript provides an effective solution to your vaccine needs, screening and optimizing parameters involved in regulating vaccinal gene expression and immunogenicity.
Customized solutions:
The optimization conditions can be tailored to meet the specific needs of even the most esoteric project.
Related Resources
 Use FREE Rare Codon Analysis Tool to check if your gene needs to be optimized.
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Optimization Parameters
OptimumGene™ algorithm optimizes a variety of parameters that
are critical to transcription, translation and protein folding:
Transcriptional Efficacy:
- GC content
- CpG dinucleotides content
- Cryptic splicing sites
- Negative CpG islands
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SD sequence TATA boxes Terminal signal |
Translation Efficiency:
- Codon usage bias
- GC content
- mRNA secondary structure
- Premature PolyA sites
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RNA instability motif (ARE) Stable free energy of mRNA Internal chi sites and ribosomal binding sites |
Protein Refolding:
- Codon usage bias
- Interaction of codon and anti-codon
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Codon-context RNA secondary structures |
Selection Volume
OptimumGene™ produces over 10,000 candidate sequences during round one alone. A complex sorting matrix then selects for the best sequence for each specific customized request.
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OptimumGene™ History of Successful Codon Optimization
GenScript has accumulated years of experience in codon optimization and gene synthesis. Our codon optimization system has optimized over 50,000 sequences in almost all major expression systems. Many top pharmaceutical companies are our customers.
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Fig. 1. Based on our historical records, the OptimumGene™ codon optimization has optimized over 50,000 sequences in all major expression systems.
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Customer Testimonial
"I think GenScript provides absolutely excellent products in a timely fashion. I have primarily used genscript to order codon optimized artificial genes. Ordering artificial genes on their website or via Email makes this especially facile because I can simply inquire about a gene, get a quote and then get a PO#. The whole process is fast and the turnaround time for obtaining the finished product is usually less than a month. I also think their technical support are wonderful people, they have been most helpful in facilitating the process. I am so impressed with them that I have recommended them to several of my colleagues."
― Dr. Maria Schumacher, The University of Texas. M.D. Anderson Cancer Center
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Newest List of Publications Cited the Use of OptimumGene™ Codon Optimization
Sa Kan Yoo, Taylor W. Starnes, Qing Deng & Anna Huttenlocher. Lyn is a redox sensor thatmediates leukocyte wound attraction in vivo. Nature. Dec 2011; 480(01):109-112
Guillaume Bouvignies, Pramodh Vallurupalli, D. Flemming Hansen, Bruno E. Correia, Oliver Lange, Alaji Bah, Robert M. Vernon, Frederick W. Dahlquist, David Baker & Lewis E. Kay. Solution structure of aminor and transiently formed state of a T4 lysozyme mutant. Nature. Sep 2011; 477(01):111-114
D H Aggen, A S Chervin, T M Schmitt, B Engels, J D Stone, S A Richman, K H Piepenbrink, B M Baker, P D Greenberg, H Schreiber andD M Kranz. Single-chain VαVβ T-cell receptors function without mispairing with endogenous TCR chains. Gene Therapy. July 2011
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Case Study
Proven increase in protein expression through optimizing a variety of parameters
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Fig. 1. GenScript OptimumGene™ codon optimization genes increased the yield of expression (8 out of 10 genes) and the degree of solubility in some cases (6 out of 10 genes) compared to the native genes.
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Reference: Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study. Protein Expr Purif. May 2008; 59(1): 94-102
Significant Increase Drug Target Proteins Expression Level in E.coli.
Figures below manifested the effectiveness of the OptimumGene™ technology in increasing drug target proteins expression levels for two different genes expressed in E.coli.
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Note: GenScript will not claim any rights whatsoever over any intellectual property or technical protocols involved in OptimumGeneTM codon optimization and gene synthesis services such as gene sequences or any materials submitted by the customer. GenScript will not release such information to any third party without customer consent. Sensitive information is only disclosed to the personnel responsible for the successful operation of the project on a need-to-know basis. GenScript will sign a non-disclosure or confidentiality agreement to ensure customers' full rights over IP for the optimized and synthesized genes if needed.
Quotation and Ordering
Orders can be placed by phone, email, fax, or online with a formal PO
(Purchase Order) or credit card. Our gene service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.
For batch orders, please send us gene sequences in FASTA format or fill out and upload the Quick Quotation Request Form. Our Ph.D level representatives will help manage your projects from sequence design, optimization, synthesis, cloning and beyond without any additional charges (Certain Terms and Conditions Applies).
By submitting this form, you agree that
- GenScript customer service personnel will have access to all the information on the form, we will input all the information into our system, GenScript will handle the information according to our confidentiality agreement with client.
- There is no fee charged by us to design or verify the cloning strategy, sequences and enter them into our system for you. You agree that there will be process time to verify and enter the sequences into system. For regular orders, we will try our best to process within 24 hours.
- You agree that you have provided the information as accurate and complete as you can, you also agree that you will examine the sequences and other information within the order summary and confirm if they are correct. GenScript will try our best to make sure there are no errors or misunderstandings for your orders
- You agree that GenScript can contact you and request or verify any information that is needed to proceed the quote if GenScript thinks the information on the form is inaccurate or incomplete. You will be responsible to provide all information that is needed to process the quote.
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