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Case Study—Large-scale Endotoxin Free Protein Production


GenScript's protein team has extensive experience in bacterial expression and purification, protein refolding, solubility enhancement, endotoxin removal, and large-scale production.

In a recent case, we have obtained 108 mg A fusion protein with >90% purity from 20 L E. coli expression and two-step purification. The concentration is 4.02 mg/ml and the endotoxin level is lower than 0.8 EU/ug.

endotoxin free protein production

  Fig. 1 SDS-PAGE analysis of the expression of A fusion protein   Fig. 2 SDS-PAGE analysis of the first step purification of A fusion protein  
  Lane M: Smart Broad-Range Protein Standard (Genscript, Cat.No. MM0906)   Lane 1: Supernatant fraction Lane 3: Wash  
Lane 2: Flow through fraction Lane 4–5: Elution  
  Lane 1: Lysate of cells without induced   Lane M: Smart Broad-Range Protein Standard (Genscript, Cat.No. MM0906)  
  Lane 2–4: Lysate of cells induced  
         
  Fig.3 SDS-PAGE analysis of the second step purification of A fusion protein   Fig. 4 SDS-PAGE analysis of the purity of A fusion protein  
Lane 1: A fusion protein
Lane M: Smart Broad-Range Protein Standard (Genscript, Cat.No. MM0906)
  Lane 1: Specimen fraction Lane 2–9: Elution    
Lane M: Smart Broad-Range Protein Standard (Genscript, Cat.No. MM0906)

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