| Product Name |
293/MCH1 Stable Cell Line |
Full Name |
Human Recombinant Melanin-concentrating Hormone Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
The protein encoded by this gene is a member of the G protein-coupled receptor family 1. It is also an integral plasma membrane protein that binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Melanin-concentrating hormone (MCH) is an important neuropeptide hormone involved in multiple physiological processes. Peptide derivatives of MCH have been developed as tools to aid researches that involve potent radioligands, receptor selective agonists, and potent antagonists. These tools have been used to further understand the role of MCH's physiology primarily in rodents. |
Freeze Medium |
45% culture medium,45% FBS, and 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for MCH1 receptor |
Application Examples |
Figure 1. MCH-induced concentration-dependent stimulation of intracellular calcium mobilization in 293/MCH1 and 293 cells. The cells were loaded with Calcium-4 prior to stimulation with a MCH1 receptor agonist, MCH. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of MCH (Mean ± SD, n = 2). The EC50 of MCH on MCH1 in 293 cells was 2.9 nM. The S/B of MCH on MCH1in 293 cells was 7. |
TECHNICAL MANUAL: 10020_20090630000207.PDF (PDF)
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