| Product Name |
CHO-K1/M4/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Muscarinic Acetylcholine Receptor M4 Stable Cell Line |
|
Species |
Human |
Description |
Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M4 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M4 with Gα15 in the CHO-K1 which supports high levels of recombinant M4 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway. |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for M4 receptor |
Application Examples |
Figure 1. Oxotremorine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an M4 receptor agonist, Oxotremorine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Oxotremorine (Mean ± SD, n = 2). The EC50 of Oxotremorine on M4 co-expressing with Gα15 in CHO-K1 cells was 14 nM. The S/B of Oxotremorine on M4 co-expressing with Gα15 in CHO-K1 cells was 28. |
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TECHNICAL MANUAL: 12909_20100610033339.PDF (PDF)