| Product Name |
CHO-K1/ADORA2A/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Adenosine A2A Receptor Stable Cell Line |
|
Species |
Human |
Description |
The adenosine receptors ADORA2A is Gs-coupled GPCRs expressed in the thymus gland, heart, lung, kedney, brain, platelets, spleen and leukocytes. ADORA2A down-regulates chemokine receptor function and inhibits platelet aggregation. ADORA2A antagonists may be useful as therapy for Parkinson's disease. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml zeocin, 100 μg/ml Hygromycin B
|
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADORA2A receptor |
Application Examples |
Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADORA2A/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADORA2A receptor agonist, NECA. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of NECA (Mean ± SD, n = 2). The EC50 of NECA on ADORA2A co-expressing with Gα15 in CHO-K1 cells was 8.8 nM. The S/B of NECA on ADORA2A co-expressing with Gα15 in CHO-K1 cells was 4. |
Document-EXAMPLE: 12961_20100617214846.JPG (JPG)
TECHNICAL MANUAL: 13037_20100710001612.PDF (PDF)