| Product Name |
CHO-K1/ADRB1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant ADRB1 Adrenoceptors Stable Cell Line |
|
Species |
Human |
Description |
The Ã-adrenergic receptors are linked to G proteins. The Ã-receptor has three known subtypes. Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction. Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation. Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd，2006). In the human heart, beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance. Changes in betaAR play an important role in chronic heart failure (CHF). Thus, due to increased sympathetic activity in CHF, betaAR are chronically (over) stimulated, and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE，2006). |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADRB1 receptor |
Application Examples |
Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRB1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADRB1 receptor agonist, Epinephrine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on ADRB1 co-expressing with Gα15 in CHO-K1 cells was 240 nM. The S/B of Epinephrine on ADRB1 co-expressing with Gα15 in CHO-K1 cells was 17. |
TECHNICAL MANUAL: 10035_20090630021556.PDF (PDF)
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