| Product Name |
CHO-K1/CCKA/Gα15 Stable Cell Line |
Full Name |
Human Recombinant CCKA Cholecystokinin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Cholecystokinin (CCK)-A and CCK-B receptors are highly homologous members of the seven transmembrane domain G-protein-coupled receptor super-family. Peptides in the cholecystokinin (CCK) family have a variety of biological functions in the central and peripheral nervous systems as well as in the gastrointestinal tract. The CCKA receptor has a more limited distribution with the highest densities in the hypothalamic nuclei, areas of the hippocampus, the septum, dorsal motor vagal nucleus, and interpeduncular nucleus of the brain stem. It also occurs in numerous gastro-intestinal tissues. Binding of ligands to CCK1 stimulates mobilization of intracellular calcium by activation of Gq/11. CCKA receptors affect satiety, pancreatitis, and gut motility and have growth-promoting effects on some tumors. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CCKA receptor |
Application Examples |
Figure 1. CCK-8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKA/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a CCKA receptor agonist, CCK-8. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of CCK-8 (Mean ± SD, n = 2). The EC50 of CCK-8 on CCKA co-expressing with Gα15 in CHO-K1 cells was 0.013 nM. The S/B of CCK-8 on CCKA co-expressing with Gα15 in CHO-K1 cells was 102. |
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