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M00441
Smart Advanced Broad-Range Protein Standard
250 μl
$35
Product Name Smart Advanced Broad-Range Protein Standard
Full Name
Smart Advanced Broad-Range Protein Standard
Documents
Document-DATASHEET: 13028_20100702070115.PDF (PDF)
Document-EXAMPLE: 13030_20100712023140.JPG (JPG)
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Reference
Description
GenScript’s Smart Advanced Broad-Range Protein Standard is used to accurately estimate the molecular weight of protein samples on SDS-PAGE. The standard consists of seven purified recombinant proteins ranging from 30 to 200kDa (30 kDa, 40 kDa, 60 kDa, 80 kDa, 100 kDa, 150 kDa, 200 kDa), of which six are unstained and one (30 kDa) is prestained with blue dye. The standard is supplied in a ready-to-use format and can be visualized with Coomassie blue or silver staining. The band of 100kDa is double intensified to serve as reference.
Concentration
2μg/5μl of 30kDa protein, 1μg/5μl of 100kDa protein and 0.5μg/5μl of the rest proteins in 50 mM Tris-HCl (pH 6.8), 5 mM EDTA, 10 mM DTT, 10% glycerol, 1% SDS and 0.01% phenol red.
Recommended loading volume
5 μl per lane is recommended to load on mini gel.
Storage
The standard can be stored at -20°C for 12 months. It is recommended to aliquot the standard into small volume.